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عناصر تنظیمی بالادست، ژنهای هدف بالقوه و الگوی بیان سه miRNA پاسخدهنده به تنش خشکی در دو رقم انگور
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اعظم مؤیدی نژاد  ، بهروز محمد پرست* ، قاسم حسینی سالکده ، احسان محسنی فرد ، محمد علی نجاتیان |
| پژوهشکده انگور و کشمش، دانشگاه ملایر، ملایر ، bmparast2013@gmail.com |
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چکیده: (11682 مشاهده) |
میکروRNAها (miRNAs) به‌عنوان گروهی از RNAهای کوچک غیرکدکننده نقش مهمی در تنظیم رشد، نمو و پاسخ گیاهان به تنش‌های مختلف ایفا می‌کنند. در این تحقیق بیان سه miRNA پاسخدهنده به خشکی (miR160 ، miR159 و (miR169 با استفاده ازqRT-PCR در دو رقم انگور متحمل (یاقوتی) و حساس به خشکی (بیدانه‌سفید) تحت شرایط تنش خشکی مورد مقایسه قرار گرفت. به‌منظور شناسایی عناصر تنظیمی بالقوه در پروموتر miRNAهای مورد بررسی، توالی بالادست پیش ساز این miRNAها توسط پایگاه دادهای PlantCARE مورد آنالیز قرار گرفت. موتیفهای مرتبط با خشکی مانند ABREها و MBSها در مناطق تنظیمی miRNAها شناسایی شدند. سه فاکتور رونویسی مرتبط با سیگنالینگ هورمونهای گیاهی اسید آبسیزیک (ABA) و اکسین به‌عنوان مهمترین ژنهای هدفmiRNA های مورد بررسی، شناسایی شدند. الگوی بیان miRNAهای مورد بررسی بسته به نوعmiRNA و نوع رقم، متفاوت بود، به‌طوری‌که بیان miRNA159 تحت تأثیر تنش در رقم بیدانه‌سفید بدون تغییر بود و در رقم یاقوتی افزایش یافت اما بیان miRNA160 وmiRNA169 در دو رقم مورد بررسی بهصورت معکوس تغییر یافت. پروفایل بیان وابسته به ژنوتیپ (رقم) نشان داد که پاسخ miRNA ها به تنش خشکی در میان ژنوتیپ‌های خیلی نزدیک با حساسیت متفاوت به تنش نیز متفاوت است. به‌طورکلی با توجه به نقش ژنهای هدف بالقوه miRNA های مورد بررسی، به‌نظر می‌رسد که تغییر بیان miRNAهای ارزیابی‌شده درنهایت به تحمل بهتر تنش خشکی در رقم یاقوتی منجر میشود.
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| واژه‌های کلیدی: بیدانه‌سفید، خشکی، ژن هدف، عناصر تنظیمی، یاقوتی |
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متن کامل [PDF 683 kb]
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نوع مطالعه: پژوهشي |
موضوع مقاله:
ژنتیک مولکولی
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| ارسال پیام به نویسنده مسئول |
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Moayedinezhad A, Mohammadparast B, Hosseini Salekdeh G, Mohsenifard E, Nejatian M A. Upstream Regulatory Elements, Potential Targets and Expression Patterns of Three Drought Responsive miRNAs in Two Grapevine Cultivars. pgr 2019; 6 (1) :115-126 URL: http://pgr.lu.ac.ir/article-1-152-fa.html
مؤیدی نژاد اعظم، محمد پرست بهروز، حسینی سالکده قاسم، محسنی فرد احسان، نجاتیان محمد علی. عناصر تنظیمی بالادست، ژنهای هدف بالقوه و الگوی بیان سه miRNA پاسخدهنده به تنش خشکی در دو رقم انگور. پژوهش های ژنتیک گیاهی. 1398; 6 (1) :115-126 URL: http://pgr.lu.ac.ir/article-1-152-fa.html
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