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:: Volume 5, Issue 1 (2018) ::
pgr 2018, 5(1): 77-86 Back to browse issues page
Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks
Shahin Jahangirzadeh Khiavi * , Zabihollah Zamani , Mohamadreza Fatahi , Masoumeh Ashourpour
Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran , sh.jahangirzadeh@ areeo.ac.ir
Abstract:   (12345 Views)
The chloroplastic DNA of 64 accessions of apple (Malus spp.) (54 Iranian genotypes, five commercial cultivars and five rootstocks) were analyzed to reveal their haplotypes by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Approximately 4320 bp of the chloroplast genome was analyzed, by using two chloroplast universal primer pairs and two restriction enzymes (EcoRI and MseI). All the mutations detected were insertion-deletions been in the range between 10-40 bp. The combination of all the mutations resulted to six haplotypes (H1, H2, H3, H4, H5 and H6) in the studied apple accessions. It was defined that the Iranian apple rootstock 'Gami-Almasi' had a specific haplotype form all the other studied accessions. It was approved that cpDNA diversity data can be considered for phylogenetic studies in this plant and the polymorphism determined in cytoplasmic genome by PCR-RFLP method can help to understand the maternal inheritance in apple.
Keywords: Chloroplast universal primer, Restriction enzyme, Malus Spp, Haplotype, PCR-RFLP
Full-Text [PDF 565 kb]   (1336 Downloads)    
Type of Study: Research | Subject: Plant genetics
Accepted: 2019/03/31
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Jahangirzadeh Khiavi S, Zamani Z, Fatahi M, Ashourpour M. Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks. pgr 2018; 5 (1) :77-86
URL: http://pgr.lu.ac.ir/article-1-124-en.html


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Volume 5, Issue 1 (2018) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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