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:: دوره 4، شماره 2 - ( 1396 ) ::
جلد 4 شماره 2 صفحات 16-1 برگشت به فهرست نسخه ها
بیان ژن cryIAb توسط پیشبر القا شونده با زخم ( MPI) به منظور افزایش مقاومت به آفت شب‌پره (Tuta absoluta) در گوجه فرنگی
زهرا حاجی احمدی ، رضا شیرزادیان خرم اباد* ، محمود کاظم زاد ، محمد مهدی سوهانی
، r.shirzadian@guilan.ac.ir
چکیده:   (14772 مشاهده)
آفت شب­پره گوجه­فرنگی (Tuta absoluta) از جمله آفات مهم گوجه­فرنگی در کشور ایران به شمار می­رود. تغذیه این آفت موجب خسارت %100-50 به میوه گوجه­فرنگی در جهان شده است. ژنcryIAb  به بسیاری از گیاهان از جمله ذرت منتقل و موجب حفاظت آن­ها در مقابل حمله کرم ساقه­خوار ذرت شده است. در اکثر مطالعات از پیشبرهای دائمی مانند CaMV35S در فرآیند انتقال ژن به گیاهان استفاده می­شود که این امر به دلیل مصرف دائمی انرژی گیاه موجب تغییر در مسیرهای متابولیسمی آن می­گردد. پیشبر القا شونده با زخم MPI (Maize Protease Inhibitor) دارای قدرت و کارایی بالاتری از پیشبر CaMV35S است. بنابراین در پژوهش حاضر، برای اولین بار گیاهان گوجه­فرنگی تراریخت (رقم فلات) حاوی ژن cryIAb تحت پیشبر MPI تولید شد. پیشبرMPI از گیاه ذرت جدا و در ناقل بیانی pPZP122 جایگزین پیشبرCaMV35s  گردید. پس از جداسازی ژنcryIAb  از ناقل pCIB4421، سازه pPZP122:MPI:CryIAb ساخته شد و به سویه AGL1 آگروباکتریوم منتقل شد. تراریخته نمودن گوجه­فرنگی بصورت In planta انجام و گزینش اولیه گیاهان ترایخت در محیط کشت­های حاوی جنتامایسین انجام شد. حضور ژن cryIAb در گیاهچه­های مقاوم نسل اول و دوم به ترتیب %5/62 و % 58/75 با PCR مورد تایید قرار گرفت. حضور پروتئین در لاین­های تراریخت نسل دوم با استفاده از روش لکه­گذاری نقطه­ای پروتئین و به کمک پادتن اختصاصی چند دودمانی پروتئین CryIAb، مورد تایید قرار گرفت. نتایج زیست سنجی گیاهان تراریخته نسل دوم با استفاده از آفت توتا، مقاومت بهبود یافته گیاهان تراریخته علیه آفت را نشان دادند. بنابراین پیشبر القا شونده با زخم MPI می­تواند برای ساخت سازه­های حاوی ژن­های کد کننده پروتئین حشره­کش (مانند انواع مختلف cry) و تراریختی سایر گیاهان مفید باشد تا ژن مورد نظر صرفا در زمان حمله آفت بیان شود.
واژه‌های کلیدی: پیشبر القا شونده توسط زخم، توتا، ژن cryIAb، In planta
متن کامل [PDF 983 kb]   (2768 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: ژنتیک گیاهی
پذیرش: 1396/9/3
فهرست منابع
1. Adeli, N. and Ghareyazie, B. (2013). Comparison between the impact of transgenic insect resistant Crop plants and their traditional counterparts on human health and the environment. Genetic Engineering and Biosafety Journal, 1: 1-28 (In Persian).
2. Amako, Y., Yamamoto, T. and Nagai, H. (1994). The119Sn Mössbauer effect in RMn6Sn6 compounds (R= Gd, Y). Hyperfine Interactions, 94: 1897-1901. [DOI:10.1007/BF02063714]
3. Angenon, G., Dillen, W. and Van Montagu, M. (1994). Antibiotic resistance markers for plant transformation In: Plant Molecular Biology Manua (Geert Angenon, G., Dillen, W. and Montagu, M.V., Eds), pp. 125-137, Springer, Chapman and Hall, London, UK. [DOI:10.1007/978-94-011-0511-8_9]
4. Baniameri, V. and Cheraghian, A. (2011). The current status of Tuta absoluta in Iran In: International Symposium on Management of Tuta absoluta, pp. 16-18, Agadir, MOR.
5. Behzadirad, M., Naghavi, M.R., Abbasi, A. and Dastmalchi, T. (2009). Phytoremediation, especial solution of biotechnology in environmental protection. Journal of Biosafety, 2: 43-50.
6. Breitler, J.C., Cordero, M.J., Royer, M., Meynard, D., San Segundo, B. and Guiderdoni, E. (2001). The− 689/+ 197 region of the maize protease inhibitor gene directs high level, wound-inducible expression of the cry1B gene which protects transgenic rice plants from stemborer attack. Molecular Breeding, 7: 259-274. [DOI:10.1023/A:1011609128319]
7. Breitler, J.C., Vassal, J.M., Del-Mar-Catala, M., Meynard, D., Marfà, V., Melé, E., Royer, M., Murillo, I., San Segundo, B. and Guiderdoni, E. (2004). Bt rice harbouring cry genes controlled by a constitutive or wound‐inducible promoter: protection and transgene expression under Mediterranean field conditions. Plant Biotechnology Journal, 2: 417-430. [DOI:10.1111/j.1467-7652.2004.00086.x]
8. Campos, M.R., Rodrigues, A.R.S., Silva, W.M., Silva, T.B.M., Silva, V.R.F., Guedes, R.N.C. and Siqueira, H.A.A. (2014). Spinosad and the tomato borer Tuta absoluta: a bioinsecticide, an invasive pest threat, and high insecticide resistance. PloS One, 9(8), (doi.org/10.1371/journal.pone .0103235). [DOI:10.1371/journal.pone.0103235]
9. Cordero, M.J., Raventós, D. and Segundo, B. (1994). Expression of a maize proteinase inhibitor gene is induced in response to wounding and fungal infection: systemic wound‐response of a monocot gene. The Plant Journal, 6: 141-150. [DOI:10.1046/j.1365-313X.1994.6020141.x]
10. Dhindsa, R.S. and Matowe, W. (1981). Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. Journal of Experimental Botany, 32: 79-91. [DOI:10.1093/jxb/32.1.79]
11. Galdino, T.V.d.S., Picanço, M.C., Morais, E.G.F.d., Silva, N.R., Silva, G.A.R.d. and Lopes, M.C. (2011). Bioassay method for toxicity studies of insecticide formulations to Tuta absoluta (Meyrick, 1917). Ciência e Agrotecnologia, 35: 869-877. [DOI:10.1590/S1413-70542011000500002]
12. Ghareyazie, B., Alinia, F., Menguito, C.A., Rubia, L.G., de Palma, J.M., Liwanag, E.A., Cohen, M.B., Khush, G.S. and Bennett, J. (1997). Enhanced resistance to two stem borers in an aromatic rice containing a synthetic cryIA(b) gene. Molecular Breeding, 3: 401-414. [DOI:10.1023/A:1009695324100]
13. Hajdukiewicz, P., Svab, Z. and Maliga, P. (1994). The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation. Plant Molecular Biology, 25: 989-994. [DOI:10.1007/BF00014672]
14. Hajiahmadi, Z., Shirzadian-Khorramabad, R., Kazemzad, M. and Sohani, M.M. (2017). In silico analysis and transient expression of wound-inducible promoter MPI in tomato (Lycopersicon esculentumMill. cv. CH). Plant Omics, 10: 118. [DOI:10.21475/poj.10.03.17.pne411]
15. Hayford, M.B., Medford, J.I., Hoffman, N.L., Rogers, S.G. and Klee, H.J. (1988). Development of a plant transformation selection system based on expression of genes encoding gentamicin acetyltransferases. Plant Physiology, 86: 1216-1222. [DOI:10.1104/pp.86.4.1216]
16. Heitz, T., Geoffroy, P., Fritig, B. and Legrand, M. (1999). The PR-6 family: proteinase inhibitors in plant-microbe and plant-insect interactions In: Pathogenesis Related Proteins in Plants (Datta, SK. and Muthukrishnan, S., Eds), 131-155, Chapman & Hall, Productivity Press, Auerbach Publications, Florida, USA. [DOI:10.1201/9781420049299.ch6]
17. Jafari, M., Norouzi, P., Malboobi, M.A., Ghareyazie, B., Valizadeh, M., Mohammadi, S.A. and Mousavi, M. (2009). Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic cry1Ab gene. Euphytica, 165: 333-344. [DOI:10.1007/s10681-008-9792-4]
18. Koiwa, H., Bressan, R.A. and Hasegawa, P.M. (1997). Regulation of protease inhibitors and plant defense. Trends in Plant Science, 2: 379-384. [DOI:10.1016/S1360-1385(97)90052-2]
19. Koziel, M.G., Beland, G.L., Bowman, C., Carozzi, N.B., Crenshaw, R., Crossland, L., Dawson, J., Desai, N., Hill, M. and Kadwell, S. (1993). Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Nature Biotechnology, 11: 194-200. [DOI:10.1038/nbt0293-194]
20. Lietti, M.M., Botto, E. and Alzogaray, R.A. (2005). Insecticide resistance in argentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Neotropical Entomology, 34: 113-119. [DOI:10.1590/S1519-566X2005000100016]
21. Martínez‐Valverde, I., Periago, M.J., Provan, G. and Chesson, A. (2002). Phenolic compounds, lycopene and antioxidant activity in commercial varieties of tomato (Lycopersicum esculentum). Journal of the Science of Food and Agriculture, 82: 323-330. [DOI:10.1002/jsfa.1035]
22. Murray, M. and Thompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8: 4321-4326. [DOI:10.1093/nar/8.19.4321]
23. Pereira, R.R., Picanço, M.C., Santana, P.A., Moreira, S.S., Guedes, R.N. and Corrêa, A.S. (2014). Insecticide toxicity and walking response of three pirate bug predators of the tomato leaf miner Tuta absoluta. Agricultural and Forest Entomology, 16: 293-301. [DOI:10.1111/afe.12059]
24. Ryan, C.A. (1990). Protease inhibitors in plants: genes for improving defenses against insects and pathogens. Annual Review of Phytopathology, 28: 425-449. [DOI:10.1146/annurev.py.28.090190.002233]
25. Stotz, H.U., Kroymann, J. and Mitchell-Olds, T. (1999). Plant-insect interactions. Current Opinion in Plant Biology, 2: 268-272. [DOI:10.1016/S1369-5266(99)80048-X]
26. Yasmeen, A., Mirza, B., Inayatullah, S., Safdar, N., Jamil, M., Ali, S. and Choudhry, M.F. (2009). In planta transformation of tomato. Plant Molecular Biology Reporter, 27: 20-28. [DOI:10.1007/s11105-008-0044-5]
27. Yinghua, S., Yan, D., Jin, C., Jiaxi, W. and Jianwu, W. (2017). Responses of the cutworm Spodoptera litura (Lepidoptera: noctuidae) to two Bt corn hybrids expressing Cry1Ab. Scientific Reports, 7: 1-13. [DOI:10.1038/srep41577]
28. Yuan, Y., Ke, X., Chen, F., Krogh, P.H. and Ge, F. (2011). Decrease in catalase activity of Folsomia candida fed a Bt rice diet. Environmental Pollution, 159:3714-3720. [DOI:10.1016/j.envpol.2011.07.015]
29. Zakerghoran, B., Memari, H.R., Ahmadi, D.N. and Siahmard, M. (2014). Cloning, Transformation and Stable Expression of a Fusion of Human Interferon Gamma and bar Genes in Tobacco Plant (Nicotiana tobaccum cv. xanthi). Plant Genetic Researches, 1: 27-36 (In Persian). [DOI:10.29252/pgr.1.1.27]
30. Zhang, S., Lian, Y., Liu, Y., Wang, X., Liu, Y. and Wang, G. (2013). Characterization of a maize Wip1 promoter in transgenic plants. International Journal of Molecular Sciences, 14: 23872-23892. [DOI:10.3390/ijms141223872]
31. Adeli, N. and Ghareyazie, B. (2013). Comparison between the impact of transgenic insect resistant Crop plants and their traditional counterparts on human health and the environment. Genetic Engineering and Biosafety Journal, 1: 1-28 (In Persian).
32. Amako, Y., Yamamoto, T. and Nagai, H. (1994). The119Sn Mössbauer effect in RMn6Sn6 compounds (R= Gd, Y). Hyperfine Interactions, 94: 1897-1901. [DOI:10.1007/BF02063714]
33. Angenon, G., Dillen, W. and Van Montagu, M. (1994). Antibiotic resistance markers for plant transformation In: Plant Molecular Biology Manua (Geert Angenon, G., Dillen, W. and Montagu, M.V., Eds), pp. 125-137, Springer, Chapman and Hall, London, UK. [DOI:10.1007/978-94-011-0511-8_9]
34. Baniameri, V. and Cheraghian, A. (2011). The current status of Tuta absoluta in Iran In: International Symposium on Management of Tuta absoluta, pp. 16-18, Agadir, MOR.
35. Behzadirad, M., Naghavi, M.R., Abbasi, A. and Dastmalchi, T. (2009). Phytoremediation, especial solution of biotechnology in environmental protection. Journal of Biosafety, 2: 43-50.
36. Breitler, J.C., Cordero, M.J., Royer, M., Meynard, D., San Segundo, B. and Guiderdoni, E. (2001). The− 689/+ 197 region of the maize protease inhibitor gene directs high level, wound-inducible expression of the cry1B gene which protects transgenic rice plants from stemborer attack. Molecular Breeding, 7: 259-274. [DOI:10.1023/A:1011609128319]
37. Breitler, J.C., Vassal, J.M., Del-Mar-Catala, M., Meynard, D., Marfà, V., Melé, E., Royer, M., Murillo, I., San Segundo, B. and Guiderdoni, E. (2004). Bt rice harbouring cry genes controlled by a constitutive or wound‐inducible promoter: protection and transgene expression under Mediterranean field conditions. Plant Biotechnology Journal, 2: 417-430. [DOI:10.1111/j.1467-7652.2004.00086.x]
38. Campos, M.R., Rodrigues, A.R.S., Silva, W.M., Silva, T.B.M., Silva, V.R.F., Guedes, R.N.C. and Siqueira, H.A.A. (2014). Spinosad and the tomato borer Tuta absoluta: a bioinsecticide, an invasive pest threat, and high insecticide resistance. PloS One, 9(8), (doi.org/10.1371/journal.pone .0103235). [DOI:10.1371/journal.pone.0103235]
39. Cordero, M.J., Raventós, D. and Segundo, B. (1994). Expression of a maize proteinase inhibitor gene is induced in response to wounding and fungal infection: systemic wound‐response of a monocot gene. The Plant Journal, 6: 141-150. [DOI:10.1046/j.1365-313X.1994.6020141.x]
40. Dhindsa, R.S. and Matowe, W. (1981). Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. Journal of Experimental Botany, 32: 79-91. [DOI:10.1093/jxb/32.1.79]
41. Galdino, T.V.d.S., Picanço, M.C., Morais, E.G.F.d., Silva, N.R., Silva, G.A.R.d. and Lopes, M.C. (2011). Bioassay method for toxicity studies of insecticide formulations to Tuta absoluta (Meyrick, 1917). Ciência e Agrotecnologia, 35: 869-877. [DOI:10.1590/S1413-70542011000500002]
42. Ghareyazie, B., Alinia, F., Menguito, C.A., Rubia, L.G., de Palma, J.M., Liwanag, E.A., Cohen, M.B., Khush, G.S. and Bennett, J. (1997). Enhanced resistance to two stem borers in an aromatic rice containing a synthetic cryIA(b) gene. Molecular Breeding, 3: 401-414. [DOI:10.1023/A:1009695324100]
43. Hajdukiewicz, P., Svab, Z. and Maliga, P. (1994). The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation. Plant Molecular Biology, 25: 989-994. [DOI:10.1007/BF00014672]
44. Hajiahmadi, Z., Shirzadian-Khorramabad, R., Kazemzad, M. and Sohani, M.M. (2017). In silico analysis and transient expression of wound-inducible promoter MPI in tomato (Lycopersicon esculentumMill. cv. CH). Plant Omics, 10: 118. [DOI:10.21475/poj.10.03.17.pne411]
45. Hayford, M.B., Medford, J.I., Hoffman, N.L., Rogers, S.G. and Klee, H.J. (1988). Development of a plant transformation selection system based on expression of genes encoding gentamicin acetyltransferases. Plant Physiology, 86: 1216-1222. [DOI:10.1104/pp.86.4.1216]
46. Heitz, T., Geoffroy, P., Fritig, B. and Legrand, M. (1999). The PR-6 family: proteinase inhibitors in plant-microbe and plant-insect interactions In: Pathogenesis Related Proteins in Plants (Datta, SK. and Muthukrishnan, S., Eds), 131-155, Chapman & Hall, Productivity Press, Auerbach Publications, Florida, USA. [DOI:10.1201/9781420049299.ch6]
47. Jafari, M., Norouzi, P., Malboobi, M.A., Ghareyazie, B., Valizadeh, M., Mohammadi, S.A. and Mousavi, M. (2009). Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic cry1Ab gene. Euphytica, 165: 333-344. [DOI:10.1007/s10681-008-9792-4]
48. Koiwa, H., Bressan, R.A. and Hasegawa, P.M. (1997). Regulation of protease inhibitors and plant defense. Trends in Plant Science, 2: 379-384. [DOI:10.1016/S1360-1385(97)90052-2]
49. Koziel, M.G., Beland, G.L., Bowman, C., Carozzi, N.B., Crenshaw, R., Crossland, L., Dawson, J., Desai, N., Hill, M. and Kadwell, S. (1993). Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Nature Biotechnology, 11: 194-200. [DOI:10.1038/nbt0293-194]
50. Lietti, M.M., Botto, E. and Alzogaray, R.A. (2005). Insecticide resistance in argentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Neotropical Entomology, 34: 113-119. [DOI:10.1590/S1519-566X2005000100016]
51. Martínez‐Valverde, I., Periago, M.J., Provan, G. and Chesson, A. (2002). Phenolic compounds, lycopene and antioxidant activity in commercial varieties of tomato (Lycopersicum esculentum). Journal of the Science of Food and Agriculture, 82: 323-330. [DOI:10.1002/jsfa.1035]
52. Murray, M. and Thompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8: 4321-4326. [DOI:10.1093/nar/8.19.4321]
53. Pereira, R.R., Picanço, M.C., Santana, P.A., Moreira, S.S., Guedes, R.N. and Corrêa, A.S. (2014). Insecticide toxicity and walking response of three pirate bug predators of the tomato leaf miner Tuta absoluta. Agricultural and Forest Entomology, 16: 293-301. [DOI:10.1111/afe.12059]
54. Ryan, C.A. (1990). Protease inhibitors in plants: genes for improving defenses against insects and pathogens. Annual Review of Phytopathology, 28: 425-449. [DOI:10.1146/annurev.py.28.090190.002233]
55. Stotz, H.U., Kroymann, J. and Mitchell-Olds, T. (1999). Plant-insect interactions. Current Opinion in Plant Biology, 2: 268-272. [DOI:10.1016/S1369-5266(99)80048-X]
56. Yasmeen, A., Mirza, B., Inayatullah, S., Safdar, N., Jamil, M., Ali, S. and Choudhry, M.F. (2009). In planta transformation of tomato. Plant Molecular Biology Reporter, 27: 20-28. [DOI:10.1007/s11105-008-0044-5]
57. Yinghua, S., Yan, D., Jin, C., Jiaxi, W. and Jianwu, W. (2017). Responses of the cutworm Spodoptera litura (Lepidoptera: noctuidae) to two Bt corn hybrids expressing Cry1Ab. Scientific Reports, 7: 1-13. [DOI:10.1038/srep41577]
58. Yuan, Y., Ke, X., Chen, F., Krogh, P.H. and Ge, F. (2011). Decrease in catalase activity of Folsomia candida fed a Bt rice diet. Environmental Pollution, 159:3714-3720. [DOI:10.1016/j.envpol.2011.07.015]
59. Zakerghoran, B., Memari, H.R., Ahmadi, D.N. and Siahmard, M. (2014). Cloning, Transformation and Stable Expression of a Fusion of Human Interferon Gamma and bar Genes in Tobacco Plant (Nicotiana tobaccum cv. xanthi). Plant Genetic Researches, 1: 27-36 (In Persian). [DOI:10.29252/pgr.1.1.27]
60. Zhang, S., Lian, Y., Liu, Y., Wang, X., Liu, Y. and Wang, G. (2013). Characterization of a maize Wip1 promoter in transgenic plants. International Journal of Molecular Sciences, 14: 23872-23892. [DOI:10.3390/ijms141223872]
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Hajiahmadi Z, Shirzadian-Khorramabad2 R, Kazemzad M, Sohani M M. Expression of cryIAb Driven by a Wound Inducible Promoter (MPI) in Tomato to Enhance Resistance to Tuta absoluta. pgr 2018; 4 (2) :1-16
URL: http://pgr.lu.ac.ir/article-1-113-fa.html

حاجی احمدی زهرا، شیرزادیان خرم اباد رضا، کاظم زاد محمود، سوهانی محمد مهدی. بیان ژن cryIAb توسط پیشبر القا شونده با زخم ( MPI) به منظور افزایش مقاومت به آفت شب‌پره (Tuta absoluta) در گوجه فرنگی. پژوهش های ژنتیک گیاهی. 1396; 4 (2) :1-16

URL: http://pgr.lu.ac.ir/article-1-113-fa.html



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