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:: دوره 8، شماره 2 - ( 1400 ) ::
جلد 8 شماره 2 صفحات 102-83 برگشت به فهرست نسخه ها
شناسایی، جداسازی و بررسی بیان خانواده ژنی هوئین در جو (Hordeum vulgare)
رضا میردریکوند*، سید سجاد سهرابی، سید محسن سهرابی، کامران سمیعی
چکیده:   (1581 مشاهده)
امروزه پپتیدهای ضدمیکروبی به‌عنوان نسل جدیدی از آنتی‌بیوتیک‌ها برای درمان بیماری‌های میکروبی در انسان، جانوران و محافظت از گیاهان در مقابل بیمارگر‌های مختلف، شناخته می‌شوند. هوئین‌ها گروهی از پپتید‌های ضدمیکروبی به‌شمار می‌روند که به‌دلیل تنوع بسیار بالا و بیان در اندام‌های مختلف گیاهی و همچنین نقش مؤثر در پاسخ به تنش‌های زیستی و غیرزیستی به‌عنوان یکی از مهم‌ترین گروه از پپتیدهای ضدمیکروبی شناخته می‌شوند. هدف مطالعه حاضر شناسایی و جداسازی ژن‌های هوئین در گیاه جو و بررسی خصوصیات و میزان بیان آن‌ها در بافت‌ها و شرایط محیطی مختلف بود. بدین منظور تمام توالی‌های پروتئینی مربوط به ژن‌های هوئین گیاهی از پایگاه NCBI دریافت شدند. پس از به‌دست آمدن توالی مورد توافق، این توالی با استفاده از ابزار tBLASTn در برابر ژنوم جو مورد هم‌ردیفی قرار گرفت. نتایج حاصل از هم‌ردیفی، سرهم‌بندی شده و توالی‌های حاصل بلاست برای تعیین چارچوب خوانش باز (ORF) کامل، دمین‌های عملکردی، سیگنال پپتید، محل تجمع سلولی، خصوصیات فیزیکوشیمیایی، فراوانی اسیدهای آمینه، فعالیت ضدمیکروبی و الگوی بیان مورد بررسی قرار گرفتند. در نهایت با استفاده از واکنش PCR توالی کدکننده کامل هوئین‌ها تکثیر و به‌صورت آزمایشگاهی تأیید شد. در این پژوهش، سیزده ژن هوئین با ORFهایی با طول متوسط 813 جفت‌باز در گیاه جو شناسایی، جداسازی و توالی‌یابی شدند. ژن‌های هوئین جداسازی شده از جو با سایر هوئین‌های گیاهی، از نظر توالی ژنی و پروتئینی و همچنین خصوصیات ساختاری و عملکردی مشابهت بالایی نشان دادند. نتایج نشان داد که هوئین‌های گیاه جو مانند سایر هوئین‌های گیاهی، بدون اینترون و یا دارای تنها یک اینترون هستند و وجود سیگنال پپتید ترشحی، تجمع خارج سلولی، وجود دمین‌های عملکردی ChtBD1 در همه آن‌ها و دمین عملکردی Lyz-like در برخی از آ‌ن‌ها، تأیید شد. همچنین نتایج نشان داد که فعالیت ضدمیکروبی و بیان حداکثری در ریشه و اندام‌های زایشی و همچنین بیان در پاسخ به تنش‌های زیستی و غیرزیستی از دیگر ویژگی‌های این پروتئین‌ها است. یافته‌های مطالعه حاضر درک ما را در مورد اثر ژن‌های هوئین در فرآیندهای زیستی گیاه مانند رشد و نمو و همچنین پاسخ به تنش‌های زیستی و غیرزیستی افزایش می‌دهد.
واژه‌های کلیدی: بیان ژن، تنش‌های زیستی و غیرزیستی، دمین عملکردی ChtBD1، هوئین
متن کامل [PDF 1675 kb]   (263 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: ژنتیک مولکولی
دریافت: 1400/8/12 | پذیرش: 1400/11/27
فهرست منابع
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Mir Derikvand R, Sohrabi S S, Sohrabi S M, Samiei K. Identification, Isolation and Expression Analysis of Hevein gene Family in Barley (Hordeum vulgar). pgr. 2022; 8 (2) :83-102
URL: http://pgr.lu.ac.ir/article-1-231-fa.html

میردریکوند رضا، سهرابی سید سجاد، سهرابی سید محسن، سمیعی کامران. شناسایی، جداسازی و بررسی بیان خانواده ژنی هوئین در جو (Hordeum vulgare). پژوهش های ژنتیک گیاهی. 1400; 8 (2) :102-83

URL: http://pgr.lu.ac.ir/article-1-231-fa.html



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