[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 2, Issue 1 (2015) ::
pgr 2015, 2(1): 1-10 Back to browse issues page
Gene Expression Pattern of Key Genes in Menthol Biosynthesis Pathway in Different Organs of Peppermint (Mentha piperita)
Soheila Afkar * , Ghasem Karimzadeh , Mokhtar Jalali Javaran
Assistant Professor, Department of Plant Breeding and Biotechnology, Payame Noor University of Lorestan, Khoramabad, Iran
Abstract:   (25130 Views)

Peppermint (Mentha piperita L., Lamiaceae) is one of the most important medicinal and aromatic plants. Menthol is the major monoterpene constituent of peppermint’s essential oil, which is used for pharmaceuticals, cosmetics and food industries. This work was aimed to study the expression pattern of important genes (Pr, Mfs, Ls) in menthol biosynthesis pathway in different plant’s organs (leaf, flower and stem) at the beginning of flowering stage. The peppermint plants were supplied from Iranian Institute of Medicinal Plants and were transferred into a greenhouse in pots. Analysis of variance showed that there is a significant difference between leaf, flower and stem, with respect to mRNA transcript level. The results of this study indicated that the level of gene expression of Pr, Mfs and Ls were altered in the different organs. There are direct relationship between the content of menthone and menthofuran and level of gene expressions of Pr and Mfs genes in flower and leaf respectively. It can be concluded that probably the expression of Mfs and Pr genes in these organs may be regulated at transcriptional level.

Keywords: Pulegone reductase, Limonene synthase, Menthofuran synthase, menthol, Mentha piperita
Full-Text [PDF 579 kb]   (4535 Downloads)    
Type of Study: Research | Subject: Plant improvement
References
1. Aflatuni, A. (2005). The yield and essential oil content of mint (Mentha ssp.) in northern ostrobothnia. University of Oulu, Finland.
2. Afkar, S. (2013). Study of important genes (Pr, Mfs, Ls) in menthol biosynthesis pathway in response to methyl jasmonate in peppermint (Mentha piperita). Ph.D. Thesis, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran (In Persian).
3. Anonymous (2003). WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants, p.1. Geneva. http://apps.who.int/medicinedocs/en/d/Js4928e"e.
4. Baytop, T. (1999). Therapy with medicinal plants in Turkey (past and present), 2nd edn, Nobel Tıp Basımevi: Istanbul, Turkey.
5. Brown, D. (1995). Encyclopaedia of herbs and their uses. Dorling Kindersley, London, UK.
6. Clark, R.J. and Menary, R.C. (1980). Environmental effects on peppermint (Mentha piperita L.). I. Effect of day length, photon flux density, night temperature and day temperature on the yield and composition of peppermint oil. Australian Journal of Plant Physiology, 7: 685-692.
7. Cowan, M.M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12: 564-582.
8. Croteau, R. and Gershenzon, J. (1994). Genetic control of monoterpen biosynthesis in mint (Mentha: Lamiaceae). Recent Advances in Phytochemistry, 28: 193-229.
9. Croteau, R.B., Davis, E.M., Ringer, K.L. and Wildung, M.R. (2005). Menthol biosynthesis and molecular genetics. Naturwissenschaften, 92: 562-577.
10. Foster, S. (1996). Peppermint, Mentha piperita. American Botanical Council - Botantical Series, 306: 3-8.
11. Gershenzon, J., McConkey, M.E. and Croteau, R.B. (2000). Regulation of monoterpene accumulation in leaves of peppermint. Plant Physiology, 122(1): 205-214.
12. Ghannadnia, M., Haddad, R., Zarinkamar, F. and Sharifi, M. (2011). Different expression of limonene synthase gene in organs and developmental stages of cumin (Cuminum cyminum L.). Iranian Journal of Medicinal and Aromatic Plants, 27(3): 495-508 (In Persian).
13. Gobert, V., Moja, S., Colson, M. and Taberlet, P. (2002). Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers. American Journal of Botany, 89 (12): 2017-2023.
14. Gomez-Galera, S., Pelacho, A.M., Capell, T. and Christou, P. (2007). The genetic manipulation of medicinal and aromatic plants. Plant Cell Report, 26(10): 1689-1715.
15. Hendriks, H. (1998). Pharmaceutical aspects of some Mentha herbs and their essential oils. Perfum Flavor, 23: 15-23.
16. Huns, K. (1997). Industrial utilization of medicinal and aromatic plants. Acta Horticulture, 503: 177-192.
17. Kim, O.T., Bang, K.H., Shin, Y.S., Lee, M.J., Jung, S.J., Hyun, D.Y., Kim, Y.C., Seong, N.S., Cha, S.W. and Hwang, B. (2007). Enhanced production of asiaticoside from hairy root cultures of Centella asiatica L. urben elicited by methyl jasmonate. Plant Cell Report, 26: 1941-1949.
18. Kumar, B., Mali, H. and Gupta, E. (2014). Genetic variability, character association, and path analysis for economic traits in menthofuran rich half-sib seed progeny of Mentha piperita L. BioMed Research International, 2014: 1-7.
19. Livak, K.J. and Schmittgrn, T.D. (2001). Analysis of relative gene epresson data using real-time quantitative PCR and 2 -ΔΔCt method. Methods, 25: 402-408.
20. Mahmoud, S. S. and Croteau, R. B. (2003). Menthofuran regulates essential oil biosynthesis in peppermint by controlling a downstream monoterpene reductase. Proceedings of the National Academy of Sciences, 100(24): 14481-14486.
21. Mahmoud, S.S., Williams, M. and Croteau, R. (2004). Cosuppression of limonene-3-hydroxylase in peppermint promotes accumulation of limonene in the essential oil. Phytochemistry, 65(5): 547-554.
22. McCaskill, D. G., Gershezon, J. and Croteau, R. B. (1992). Morphology and monoterpene biosynthetic capabilities of secondary cell vlusters isolated from glanular trichomes of peppermint (Mentha piperita L.). Planta, 187(4): 445-454.
23. McKay, D.L. and Blumberg, J.B. (2006). A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.). Phytotherapy Research, 20: 619-633.
24. Niu, Y., Luo, H., Sun, C., Yang, T.J., Dong, L., Huang, L. and Chen, S. (2014). Expression profiling of the triterpene saponin biosynthesis genes FPS, SS, SE, and DS in the medicinal plant Panax notoginseng. Gene, 533(1): 295-303.
25. Omidbaigi, R. (2005). Production and processing of medicinal plants, Astan Qouds Razavi, Mashahd, Khorasane Razavi, IR (In Persian).
26. Rajeswara Rao, B.R., Syamasundar, K.V., Rajput, D.K., Nagaraju, G. and Adinarayana, G. (2012). Biodiversity, conservation and cultivation of medicinal plants. Journal of Pharmacognosy and Phytochemistry, 3(2): 59-62.
27. Rios-Estepa, R., Turner, G., Lee, J.M., Croteau, R.B. and Lange, B.M. (2008). A systems biology approach identifies the biochemical mechanisms regulating monoterpenoid essential oil composition in peppermint. Biochemistry, 105(8): 2818-2823.
28. Tahsili, J., Sharifi, M., Behmanesh, M. and Ziaei, M. (2010). Gene expression of eugenol O -methyl transferase and components of essential oils in (Ocimum basilicum L.) at different stages of growth. Iranian Journal of Biology, 23(1): 18-25 (In Persian).
29. Velikova, V., Loreto, F., Tsonev, T., Brilli, F. and Edreva, A. (2006). Isoprene prevents the negative consequences of high temperature stress in Platanus orientalis leaves. Functional Plant Biology, 33: 1-10.
30. Verma, R.S., Rahman, L., Vermal, R.K., Chauhan, A., Yadav, A.K. and Singh, A. (2010). Essential oil composition of menthol mint (Mentha arvensis) and peppermint (Mentha piperita). cultivars at different stages of plant growth from Kumaon region of Western Himalaya. Open Access Journal of Medicinal and Aromatic Plants, 1(1): 13-18.
31. Zhang, L. and Barritt, G.J. (2004). Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells, Cancer Research, 64: 8365-8373.



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Afkar S, Karimzadeh G, Jalali Javaran M. Gene Expression Pattern of Key Genes in Menthol Biosynthesis Pathway in Different Organs of Peppermint (Mentha piperita). pgr 2015; 2 (1) :1-10
URL: http://pgr.lu.ac.ir/article-1-38-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 2, Issue 1 (2015) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657