The Study of Relative Gene Expression of Key Genes of Terpene Biosynthesis in Tissues and Different Developmental Stages of Feverfew (Tanacetum parthenium) Genotypes Using Real-Time PCR

Majdi, Mohammad; Karimzade, Ghasem; Malboobi, Mohammad Ali

doi:10.29252/pgr.1.2.25

[Home ] [Archive]

[ فارسی ]

Main Menu

Home

Journal Information

Articles archive

For Authors

For Reviewers

Registration

Contact us

Site Facilities

Search in website

Receive site information

Volume 1, Issue 2 (2015)

pgr 2015, 1(2): 25-32

Back to browse issues page

The Study of Relative Gene Expression of Key Genes of Terpene Biosynthesis in Tissues and Different Developmental Stages of Feverfew (Tanacetum parthenium) Genotypes Using Real-Time PCR

Mohammad Majdi ^*

, Ghasem Karimzade

, Mohammad Ali Malboobi

Assistant Professor, Department of Agricultural Biotechnology, Research Center for Medicinal Plant Breeding and Development, Faculty of Agriculture, Kurdistan University,Sanandaj, Iran

Abstract: (26453 Views)

Feverfew (Tanacetum partheniumL. Schulz Bip.) is a medicinal herb belonging to the Asteraceae which recently raised researcher’s attention due to its medicinal value and pharmacological activities, especially as a migraine prophylaxis agent and also for treatment of cancer. Parthenolide has a sesquiterpene lactone structure which is most likely synthesized through the mevalonic acid (MVA) pathway. Recently, it has been shown that there is cross a talk between the MVA and the MEP pathways through IDP (Iosopentenyl diphosphate) exchange as a precursor for the biosynthesis of different terpenes hence, parthenolide biosynthesis could be affected by the MEP pathway as well. In the present work, the relative gene expression of two key genes of the MVA pathway, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) and germacrene A synthase (GAS) and two key genes of the MEP pathway including 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) and hydroxy-2-methyl-2-(E)-butenyl4-diphosphate reductase (HDR) were examined in leaves derived from the vegetative or generative phases, also in flowers using real time PCR. The results of our study showed that expression of these genes depend on the growth stage and genotype. Moreover, in which in the flowers and leaves derived from vegetative phase in different genotypes only the TpGAS gene expression showed a significant difference, while in the leaves derived of generative phase the relative gene expression showed a significant difference for TpHMGR, TpGAS and TpDXR.

Keywords: Tanacetum parthenium, Sesquiterpene, Gene expression, MVA pathway, Growth stage

Full-Text [PDF 714 kb] (5088 Downloads)

Type of Study: Research | Subject: Plant improvement

References

1. Arsenault, P.R., Vail, D., Wobbe, K.K., Erickson, K. and Weathers, P.J. (2010). Reproductive development modulates gene expression and metabolite levels with possible feedback inhibition of artemisinin in Artemisia annua. Plant Physiology, 154: 958-968.

2. Bouwmeester, H.J., Jan Kodde, F.W.A., Verstappen, I.G., De Kraker, J.W. and Wallaart, T.E. (2002). Isolation and characterization of two germacrene a synthase cDNA clones from chicory. Plant Physiology, 129: 134-144.

3. Brown, A.M.G., Edwards, C.M., Davey, M.R., Power, J.B. and Lowe, K.C. (1997). Effects of extracts of Tanacetum species on human polymorphonuclear leucocyte activity in vitro. Phytotherapy Research, 11: 479-484.

4. Chappell, J. (1995). Biochemistry and molecular biology of the isoprenoid biosynthesis pathway in plants. Annual Review in Plant Physiology and Plant Molecular Biology, 46: 521-547.

5. Cretnik, L., Skerget, M. and Knez, Z. (2005). Separation of parthenolide from feverfew: performance of conventional and high-pressure extraction techniques. Separation and Purification Technology, 41: 13-20.

6. Davis, E. and Croteau, R. (2000). Cyclization enzymes in the biosynthesis of monoterpenes, sesquiterpenes, and diterpenes In: Biosynthesis (Leeper, F.L. and Vederas, J.C., Eds) pp. 53-95, Springer Berlin Heidelberg, Washington, USA.

7. De Kraker, J.W., Franssen, M.C. R., De Groot, A., Konig, W.A. and Bouwmeester, H.J. (1998(. (+)-Germacrene A biosynthesis - The committed step in the biosynthesis of bitter sesquiterpene lactones in chicory. Plant Physiology, 117: 1381-1392.

8. De Kraker, J.W., Franssen, M.C.R., Joerink, M., De Groot, A. and Bouwmeester, H.J. (2002). Biosynthesis of costunolide, dihydrocostunolide, and leucodin. demonstration of cytochrome P450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory. Plant Physiology, 129: 257-268.

9. Gopfert, J.C., MacNevin, G., Ro, D.K. and Spring, O. (2009). Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes. BioMed Central Plant Biology, 9: 86-103.

10. Groenewegen, W.A. and Heptinstall, S. (1992). Progress in the medicinal chemistry of the herb, feverfew. Progress in Medicinal Chemistry, 29:217-238.

11. Ijima, Y., Davidovich-Rikanati, R., Fridman, E., Gang, D.R., Bar, E., Lewinsohn, E. and Pichersky, E. (2004). The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of basil. Plant Physiology, 136: 3724-36.

12. Livak, K.J. and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods, 25: 402-408.

13. Majdi, M., Karimzadeh, G., Malboobi, M.A., Omidbaigi, R. and Mirzaghaderi, G. (2010). Induction of tetraploidy to feverfew (Tanacetum parthenium Schulz. Bip): morphological , physiological, cytological and phytochemical changes. Hortscience, 45:16–21.

14. Majdi, M., Karimzadeh, G. and Malboobi, M.A. (2014). Spatial and developmental expression of key genes of terpene biosynthesis in feverfew (Tanacetum parthenium). Biologia plantarum, 58: 379–384.

15. Majdi, M., Charnikhova, T. and Bouwmeester, H. (2013). Genetical, developmental and spatial factors influencing parthenolide and its precursor costunolide in feverfew (Tanacetum parthenium). Industrial Crops and Products, 47: 270-276.

16. Majdi, M., Qing, L., Karimzadeh, G., Malboobi, M.A., Beekwilder, J., Cankar, K., De Vos, R., Todorović, S., Simonović, A. and Bouwmeester, H. (2011). Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip.). Phytochemistry, 72: 1739-1750.

17. Omidbaigi, R., Dadman, B. and Yavari, S. (2007). Various harvest times of Tanacetum perthenium cv. Zardband flowers affecting its oil content and compositions. Journal of Essential Oil Bearing Plants, 10: 287-291.

18. Rohmer, M., Knani, M., Simonin, P., Sutter, B. and Sahm, H. (1993). Isoprenoid biosynthesis in bacteria: a novel pathway for early steps leading to isopentenyl diphosphate. Biochemistry Journal,295: 517-524.

19. Van Klink, J., Becker, H., Andersson, S. and Boland, W. (2003). Biosynthesis of anthecotuloide, an irregular sesquiterpene lactone from Anthemis cotula L. (Asteraceae) via a non-farnesyl diphosphate route. Organic and Biomolecular Chemistry, 1: 1503-1508.

20. Williams, C. A., Hoult, J. R. S. Harborne, J. B. Greenham, J. and Eagles, J. (1995). Biologicallyactive lipophilic flavonol from Tanacetum parthenium. Phytochemistry, 38: 267-270.

‎ 10.29252/pgr.1.2.25

‎ 20.1001.1.23831367.1393.1.2.3.0

Mendeley

Zotero

RefWorks

Majdi M, Karimzade G, Malboobi M A. The Study of Relative Gene Expression of Key Genes of Terpene Biosynthesis in Tissues and Different Developmental Stages of Feverfew (Tanacetum parthenium) Genotypes Using Real-Time PCR. pgr 2015; 1 (2) :25-32
URL: http://pgr.lu.ac.ir/article-1-33-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 1, Issue 2 (2015)

Back to browse issues page

Persian site map - English site map - Created in 0.07 seconds with 38 queries by YEKTAWEB 4657