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Investigating Variation of Subunits of Gliadin Protein in Recombinant Inbred Lines Derived from Cross Between Zagros and Norstar Cultivars and Some Commercial Wheats
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Nasrin Akbari    , Siamak Alavi Kia * , Mostafa Valizadeh |
| Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Iran , ss.alavikia@tabrizu.ac.ir |
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Abstract: (190 Views) |
Due to world population incline and the increasing wheat consumption as human main staple food, as well as high amount of waste of bread which is mainly due to its low quality, the wheat breeding programs to improve bread quality are of great importance. Therefore, evaluating the wheat grains quality and the genetic variation of bread-making quality traits among lines derived from crosses becomes imperative. To this end, the gliadin protein banding pattern of 28 recombinant inbred lines, their corresponding parents and 10 other commercial cultivars were examined via A-PAGE method. The variation between and within the lines and cultivars was determined using AMOVA according to the protein bands. The results of this study revealed high variation for gliadins coding loci with total mean of 73.96%. The percentage of polymorphism was estimated to be 91.67 and 56.25 for lines and commercial cultivars, respectively. The minimum and maximum number of gliadin bands were 12 and 25 bands, respectively. Also, based on PhiPT statistics, the significant difference was observed (P<0.05) between commercial cultivars and recombinant inbred lines in terms of gliadin banding patterns. Cluster analysis and PCoA via banding pattern of gliadins led to formation of three and four distinct groups, respectively. The highest variation was observed in ω-gliadins, suggesting that they may have a role in observed variation among genotypes and their bread making-quality traits.
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| Keywords: Grain reserve protein, Gliadin, Bread wheat, Molecular markers, A-PAGE |
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Type of Study: Applicable |
Subject:
Plant improvement
Accepted: 2023/12/26
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