[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 1, Issue 1 (2014) ::
pgr 2014, 1(1): 37-50 Back to browse issues page
Investigation of Esterase and Peroxidase Isozymes in Improved and Iranian Landraces of Alfalfa and Their Relationships with Agronomic and Morphological Traits
Maryam Ahmadi * , Mustafa Valizadeh , Mahmoud Tourchi , Mohammad Moghaddam Vahed , Hossein Mohammadzadeh Jalaly
Former M.Sc. Student, Department of Plant Breeding and Biotechnology, Agriculture Faculty, University of Tabriz, Tabriz
Abstract:   (24117 Views)
For evaluation of genetic diversity among improved alfalfa varieties and Iranian landraces, 12 populations including five improved varieties (Kaysari, Kadi, Ranger, Mesmir, Seariver) and seven landraces (Gharayonje, Amozeynadin, Rahnani, Tazekand, Shazand, Hamedani, Yazdi) were evaluated using agronomic traits and enzyme markers. Thirty-five individuals of each variety were grown and analyzed in separate pots in a unbalanced completely randomized design (CRD). Analysis of variance for agronomic traits showed significant differences for most of the traits among improved and landrace varieties. For esterase and peroxidase enzymes based on presence or absence of enzyme bands (1, 0) eleven polymprphic isozyme bands were detected. For improved and landrace varieties Shanon index mean was 0.48 ± 0.246 and 0.519 ± 0.193, respectively, furthermore Nei genetic diversity index mean for improved and landraces was 0.327 ± 0.181 and 0.352 ± 0.148 respectively, suggesting no difference between improved and landrace varieties was found. Analysis of relation between isozyme markers and agronomic traits showed that there are significant differences between the presence of POX-4 and wet and dry yield in improved varieties.
Keywords: Alfalfa, Genetic diversity, Isozyme markers, Polymorphism
Full-Text [PDF 659 kb]   (2532 Downloads)    
Type of Study: Research | Subject: Plant improvement
Accepted: 2014/07/20
References
1. Abdollahi, B. (2013). Study of genetic diversity in some populations of cultivated alfalfa (Medicago sativa L.) using morphological traits. Modern Genetic, 7: 381-388 (In Persian(.
2. Bouton, J. (1982). Seedling characteristics to predict yield and total N of mature alfalfa plants. Crop Science, 22: 128-130.
3. Bullitta, S., Floris, R., Hayward, M., Loi, A., Porqueddu, C. and Veronesi, F. (1994). Morphological and biochemical variation in Sardinian populations of Medicago polymorpha L. suitable for rainfed Mediterranean conditions. Euphytica, 77: 263-268.
4. Clegg, M. (1997). Plant Genetic Diversity and the Struggel to Measure Selection. Journal of Heredity, 88: 1-7.
5. Corts, M.M. and Martinez, M.C. (2000). Variation of PGM and IDH isozymes for identification of alfalfa varieties. Euphytica, 112: 137-143.
6. Hartl, D.L. and Clark, A.G. (1989). Principles of population genetics, 2 nd edn, Sinauer associates, Massachusetts, USA.
7. Hedrick, P.W. (2005). Genetics of populations, 3th edn, Jones and Bartlett Publishers, Massachusetts, USA.
8. Kaljund, K. and Jaaska, V. (2010). No loss of genetic diversity in small and isolated populations of "Medicago sativa "subsp. falcata". Biochemical Systematics and Ecology, 38: 510-520.
9. Labdi, M., Robertson, L., Singh, K. and Charrier, A. (1996). Genetic diversity and phylogenetic relationships among the annual Cicer species as revealed by isozyme polymorphism. Euphytica, 88: 181-188.
10. Lamkey, K. and Lee, M. (1993). Quantitative genetics, molecular markers, and plant improvement. Paper presented at the Focused plant improvement: Towards responsible and sustainable agriculture Proc 10th Australian Plant Breeding Conf, Gold Coast.
11. McCoy, T.J. and Bingham, E.T. (1988). Cytology and cytogenetics of alfalfa In: Alfalfa and Alfalfa improvement (Hanson, A., Barnes, D. and Hill, R., Eds.) pp. 737-776, Agron, Monograph 29, Madison, USA.
12. Mengoni, A., Gori, A. and Bazzicalupo, M. (2000). Use of RAPD and microsatellite (SSR) variation to assess genetic relationships among populations of tetraploid alfalfa, Medicago sativa. Plant Breeding, 119: 311-317.
13. Michaud, R., Lehman, W. and Rumbaugh, M. (1988). World distribution and historical development In: Alfalfa and alfalfa improvement (Hanson, A., Barnes, D. and Hill, R., Eds.) pp.25-91, Agron, Monograph 29, Madison, USA.
14. Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, 70: 3321-3323.
15. Oleson, B. (1994). World wheat production, utilization and trade In: Wheat Production, Properties and Quality (Bushuk, W. and Rasper, V. F., Eds.) pp. 1-11, Springer, Chapman and Hall, London, UK.
16. Quiros, C.F. (1980). Identification of alfalfa plants by enzyme electrophoresis. Crop science, 20: 262- 264.
17. Simons, R. (1990). Relationships between seedling traits and mature plant yield in alfalfa. Canadian Journal of Plant Science, 69: 206-213.
18. Singh, A.K., Mishra, A. and Shukla, A. (2009). Genetic assessment of traits and genetic relationship in blackgram (Vigna mungo) revealed by isoenzymes. Biochemical Genetics, 47: 471-485.
19. Soltis, D.E. and Soltis, P.S. (1990). Isozymes in plant biology, Springer, Chapman and Hall, London, UK.
20. Tucak, M., Popovic, S., Cupic, T., Grljusic, S., Bolaric, S. and Kozumplik, V. (2008). Genetic diversity of alfalfa (Medicago spp.) estimated by molecular markers and morphological characters. Periodicum Biologorum, 110: 243-249.
21. Valizadeh, M., Mohayeji, M., Yasinzadeh, N., Nasrullazadeh, S. and Moghadam, M. (2011).Genetic diversity of synthetic alfalfa generations and cultivars using tetrasomic inherited allozyme markers. Journal of Agricultural Science and Technology, 13: 425-430.
22. Van-Xuan, D. and Jin, I.D. (2011). Relationship between esterase isozymes and some agronomic traits in F2 populations derived from the crossing of Milyang 23 and Ashahi. Journal of Crop Science and Biotechnology, 14: 11-15.
23. Veronesi, F., Brummer, E.C. and Huyghe, C. (2010). Alfalfa In: Handbook of plant breeding : Fodder Crops and Amenity Grasses (Boller, B., Posselt, U.K. and Veronesi, F., Eds.) pp. 395-437, Springer, New York, USA.
24. Veronesi, F., Rosellini, D. and Albertini, E. (2003). The use of molecular markers in alfalfa breeding. Czech Journal of Genetics and Plant Breeding, 39: 104-111.
25. Xie, C. and Mosjidis, J. (1995). Seedling-selection effects on morphological traits of mature plants in red clover. Theoretical and Applied Genetics, 91: 1032-1036.
26. Yeh, F., Yang, R. and Boyle, T. (1999). POPGENE VERSION 1.31: Microsoft Window-based Freeware for Population Genetic Analysis, University of Alberta. Edmonton Journal, AB, Canada. Downloaded from journals.lu.ac.ir at 11:26 IRDT on Monday August 21st 2017.
27. Zeidler, M. (2000). Electrophoretic analysis of plant isozymes. Biologica, 38: 7-16.



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahmadi M, Valizadeh M, Tourchi M, Moghaddam Vahed M, Mohammadzadeh Jalaly H. Investigation of Esterase and Peroxidase Isozymes in Improved and Iranian Landraces of Alfalfa and Their Relationships with Agronomic and Morphological Traits. pgr 2014; 1 (1) :37-50
URL: http://pgr.lu.ac.ir/article-1-27-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 1, Issue 1 (2014) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657