[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 9, Issue 2 (2023) ::
pgr 2023, 9(2): 123-134 Back to browse issues page
Evaluation of Some Biochemical Traits and Expression of Two Genes from the MYB Family in Two Wheat Cultivars Under Drought Stress
Saeid Navabpour * , Horeyeh Najafi
Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran , s.navabpour@gau.ac.ir
Abstract:   (4003 Views)
Environmental stress is one of the main factors that reduce the growth and performance of crops and threatening human food security. This study was conducted in order to investigate the effect of drought stress on the changes in biochemical traits and the level of expression of a MYB transcription factor gene in two wheat cultivars (Tajan and Zagros), under drought stress. The experiment was conducted as a factorial based on a completely randomized design with 3 replications. Drought treatments were applied at three levels of 40, 70 and 100% of field capacity 4 weeks after germination. Twenty days after the application of stress, leaves and roots were sampled in order to investigate the expression of MYB genes and measuring some biochemical traits. The results of examining the chlorophyll content under stress showed that the content of chlorophyll a and b decreased with increasing of stress intensity in different genotypes. The rate of reduction of chlorophyll a and b in Tajan genotype under severe stress was higher than Zagros genotype. Also, TBARM content under severe drought stress was significantly higher than moderate stress condition and this increase was seen in Tajen genotype more than Zagros genotype. qRT-PCR analysis showed that the MYB genes showed an increase in expression under drought stress. Furthermore, Zagros genotype, which is considered as a tolerant cultivar to drought stress, had a higher MYB expression level than Tajan cultivar for both genes, suggesting this cultivar for future breeding programs, also considering the importance MYB family genes during drought stress, the results can be used in molecular breeding and pyramiding breeding projects.
Keywords: Gene expression, Drought stress, Wheat, Biochemical traits
Full-Text [PDF 471 kb]   (888 Downloads)    
Type of Study: Research | Subject: Molecular genetics
References
1. Ahmed, I.M., Dai, H., Zheng, W., Cao, F., Zhang, G., Sun, D. and Wu, F. (2013). Genotypic differences in physiological characteristics in the tolerance to drought and salinity combined stress between Tibetan wild and cultivated barley. Plant Physiology and Biochemistry, 63: 49-60. [DOI:10.1016/j.plaphy.2012.11.004] [PMID]
2. Arzani, A. )2011(. Breeding Field Crops, 4th Edition. Isfahan University of Technology Publications, Isfahan, Iran (In Persian).
3. Ayala, A., Muñoz, M.F. and Argüelles, S. )2014(. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 2014: 360438. [DOI:10.1155/2014/360438] [PMID] [PMCID]
4. Bi, H., Luang, S., Li, Y., Bazanova, N., Morran, S., Song, Z., Perera, M.A., Hrmova, M., Borisjuk, N. and Lopato, S. (2016). Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis. Journal of Experimental Botany, 67(18): 5363-5380. [DOI:10.1093/jxb/erw298] [PMID] [PMCID]
5. Bouchemal, K., Bouldjadj, R., Belbekri, M.N., Ykhlef, N. and Djekoun, A. (2017). Differences in antioxidant enzyme activities and oxidative markers in ten wheat (Triticum durum Desf.) genotypes in response to drought, heat and paraquat stress. Archives of Agronomy and Soil Science, 63: 710-722. [DOI:10.1080/03650340.2016.1235267]
6. Dubos, C., Stracke, R., Grotewold, E., Weisshaar, B., Martin, C. and Lepiniec, L. (2010). MYB transcription factors in Arabidopsis. Trends in Plant Science, 15(10): 573-581. [DOI:10.1016/j.tplants.2010.06.005] [PMID]
7. Emam, Y. (2013). Cereal Cultivation. Shiraz University Press, Shiraz, IR (In Persian).
8. Esfandiari, E., Javadi, A., Shokrpour, M. and Shekari, F. (2011). The effect of salt stress on the antioxidant defense mechanisms of two wheat (Triticum aestivum L.) cultivars. Fresenius Environmental Bulletin, 20(8a): 2021-2026.
9. Fahmideh, L., Delarampoor, M.A. and Fooladvand, Z. (2020). Study of MYB transcription factor gene expression in some bread wheat cultivars of Sistan region, Iran. Plant Genetic Researches, 7(1): 181-196 (In Persian). [DOI:10.52547/pgr.7.1.11]
10. Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., and Basra, S.M.A. (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29: 185-212. [DOI:10.1051/agro:2008021]
11. Hagege, D., Nouvelot, A., Boucard, J. and Gaspar, T. (1990). Malondialdehyde titration with thiobarbiturate in plant extracts: avoidance of pigment interference. Phytochemical Analysis, 1: 86-89. [DOI:10.1002/pca.2800010208]
12. Hu, R., Sun, K., S.u, X., Pan, Y.X., Zhang, Y.F. and Wang, X.P. (2012). Physiological responses and tolerance mechanisms to Pb in two xerophils: Salsola passerina Bunge and Chenopodium album L. Journal of Hazardous Materials, 205: 131-138. [DOI:10.1016/j.jhazmat.2011.12.051] [PMID]
13. Humai, M. (2012). The Reaction of Plants to Salinity. National Irrigation and Drainage Committee of Iran Publication, Tehran, IR (In Persian).
14. Kaur, G., Singh, H.P., Batish, D.R. and Kumar, R.K. (2012). Growth, photosynthetic activity and oxidative stress in wheat (Triticum aestivum) after exposure of lead to soil. Journal of Environmental Biology, 33(2): 265.
15. Liu, Y., Liang, H., Lv, X., Liu, D., Wen, X. and Liao, Y. (2016). Effect of polyamines on the grain filling of wheat under drought stress. Plant Physiology and Biochemistry, 100: 113-129. [DOI:10.1016/j.plaphy.2016.01.003] [PMID]
16. Lobato, A.K.S., Luz, L.M., Costa, R.C.L., Santos Filho, B.G., Meirelles, A.C.S., Oliveira Neto, C.F., Laughinghouse IV, H., Neto, M.A.M., Alves, G.A.R., Lopes, M.J.S. and Neves, H.K.B. (2009). Silicon exercises influence on nitrogen compounds in pepper subjected to water deficit. Research Journal of Biological Sciences, 4(9): 1048-1055.
17. Maali-Amiri, R., Goldenkova-Pavlova, I.V., Yur'Eva, N.O., Pchelkin, V.P., Tsydendambaev, V.D., Vereshchagin, A.G., Deryabin, A.N., Trunova, T.I., Los, D.A. and Nosov, A.M. (2007). Lipid fatty acid composition of potato plants transformed with the Δ12-desaturase gene from cyanobacterium. Russian Journal of Plant Physiology, 54: 600-606. [DOI:10.1134/S1021443707050056]
18. Mao, X., Jia, D., Li, A., Zhang, H., Tian, S., Zhang, X., Jia, J. and Jing, R. (2011). Transgenic expression of TaMYB2A confers enhanced tolerance to multiple abiotic stresses in Arabidopsis. Functional & Integrative Genomics, 11: 445-465. [DOI:10.1007/s10142-011-0218-3] [PMID]
19. Mozafari, A. (2014). Evaluation the effects of plant growth promoting rhizobacteria (PGPR) on SOD, MDA and Proline content in two wheat cultivar. 20th World Congress of Soil Science. June 8-13, Jeju, Korea.
20. Naeemi, T., Fahmideh, L. and Fakheri, B. (2019). The effect of drought stress on MYB gene expression and osmotic regulator levels of five durum wheat genotypes (Triticum turgidum L.). Nova Biologica Reperta, 6: 217-228 (In Persian). [DOI:10.29252/nbr.6.2.217]
21. Navabpour, S. (2013). Induced genes expression pattern in response to drought stress in rapeseed (Brassica napus). Seed and Plant Journal, 29(3): 535-549 (In Persian).
22. Nazari Khakshoor, E., Azadi, A., Fourozesh, P., Etminan, A. and Majidi Hervan, E. (2022). Prioritization and identification of candidate genes associated with root traits under salinity stress in bread wheat (Triticum aestivum L.). Plant Genetic Researches, 9(1): 71-84 (In Persian). [DOI:10.52547/pgr.9.1.6]
23. Nezhadahmadi, A., Hossain-Prodhan, Z. and Faruq, G.)2013(. Drought tolerance in wheat. The Scientific World Journal, 1: 610-721. [DOI:10.1155/2013/610721] [PMID] [PMCID]
24. Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29(9): 45-45. [DOI:10.1093/nar/29.9.e45] [PMID] [PMCID]
25. Rahaei, M., Xue, G.P., Naghavi, M.R., Alizadeh, H. and Schenk, P.M. (2010). A MYB gene from wheat (Triticum aestivum L.) is up -regulated during salt and drought stresses and differentially regulated between salt -tolerant and sensitive genotypes. Plant Cell Reports, 29: 835-844. [DOI:10.1007/s00299-010-0868-y] [PMID]
26. Rahimi, Z., Hosseinpanahi, F. and Siosemardeh, A. (2019). Effects of drought stress on antioxidant enzymes activity and some physiological traits of drought resistant and susceptible cultivars of wheat (Triticum aestivum L.). Journal of Wheat Research, 2(1): 69-86 (In Persian).
27. Reynolds, M.P., Mujeeb‐Kazi, A. and Sawkins, M. (2005). Prospects for utilizing plant‐adaptive mechanisms to improve wheat and other crops in drought‐and salinity‐prone environments. Annals of Applied Biology, 146(2): 239-259. [DOI:10.1111/j.1744-7348.2005.040058.x]
28. Riechmann, J.L., Heard, J., Martin, G., Reuber, L., Jiang, C.Z., Keddie, J., Adam, L., Pineda, O., Ratcliffe, O.J., Samaha, R.R. and Creelman, R. (2000). Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 290(5499): 2105-2110. [DOI:10.1126/science.290.5499.2105] [PMID]
29. Russo, M.A. and Belligno, A. )2010(. Different availabilities of reduced nitrogen: Effects on oxidative stress in chicory plants, Emirates Journal of Food and Agriculture, 22(4): 250-258. [DOI:10.9755/ejfa.v22i4.4873]
30. Shao, R.X., Xin, L.F., Zheng, H.F., Li, L.L., Ran, W.L., Mao, J. and Yang, Q.H. (2015). Changes in chloroplast ultrastructure in leaves of drought-stressed maize inbred lines. Photosynthetica, 54(1): 74-80. [DOI:10.1007/s11099-015-0158-6]
31. Tabaraki, H., Fahmideh, L. and Fooladvand, Z. (2017). Study of MYB gene expression under drought stress in some bread wheat cultivars. Journal of Genetic Engineering and Biosafety, 6: 95-104 (In Persian).
32. Thirumalaikumar, V.P., Devkar, V., Mehterov, N., Ali, S., Ozgur, R., Turkan, I. and Balazadeh, S. (2018(. NAC transcription factor JUNGBRUNNEN 1 enhances drought tolerance in tomato. Plant Biotechnology Journal, 16: 354-366. [DOI:10.1111/pbi.12776] [PMID] [PMCID]
33. Xiong, H., Li, J., Liu, P., Duan, J., Zhao, Y., Guo, X., Li, Y., Zhang, H., Ali, J. and Li, Z. (2014). Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice. PloS one, 9(3): e92913. [DOI:10.1371/journal.pone.0092913] [PMID] [PMCID]
34. Yang, H., Zhao, L., Zhao, S., Wang, J. and Shi, H. )2017(. Biochemical and transcriptomic analyses of drought stress responses of LY1306 tobacco strain. Scientific Reports, 7: 1-10. [DOI:10.1038/s41598-017-17045-2] [PMID] [PMCID]
35. Zhang, C., Luo, L., Xu, W., Ledwith, V. (2008). Use of local Moran's I and GIS to identify pollution hotspots of Pb in urban soils of Galway, Ireland. Science of The Total Environment, 398: 212-221. [DOI:10.1016/j.scitotenv.2008.03.011] [PMID]
36. Zhang, L., Zhao, G., Jia, J., Liu, X. and Kong, X) .2012a(. Molecular characterization of 60 isolated wheat MYB genes and analysis of their expression during abiotic stress. Journal Experimental Botany, 63: 203-214. [DOI:10.1093/jxb/err264] [PMID] [PMCID]
37. Zhang, L., Zhao, G., Xia, C., Jia, J., Liu, X. and Kong, X. (2012b). A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. Journal of Experimental Botany, 63(16): 5873-5885. [DOI:10.1093/jxb/ers237] [PMID]
38. Zhao, Y., Cheng, X., Liu, X., Wu, H., Bi, H. and Xu, H. (2018). The wheat MYB transcription factor TaMYB31 is involved in drought stress responses in Arabidopsis. Frontiers in Plant Science, 9: 1426. [DOI:10.3389/fpls.2018.01426] [PMID] [PMCID]
39. Zhao, Y., Tian, X., Li, Y., Zhang, L., Guan, P., Kou, X., Wang, X., Xin, M., Hu, Z., Yao, Y. and Ni, Z. (2017). Molecular and functional characterization of wheat ARGOS genes influencing plant growth and stress tolerance. Frontiers in Plant Science, 8: 170. [DOI:10.3389/fpls.2017.00170]
Send email to the article author



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Navabpour S, Najafi H. Evaluation of Some Biochemical Traits and Expression of Two Genes from the MYB Family in Two Wheat Cultivars Under Drought Stress. pgr 2023; 9 (2) :123-134
URL: http://pgr.lu.ac.ir/article-1-267-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 9, Issue 2 (2023) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657