Determination of haplotype variation and relationship between desirable agronomic traits and microsatellite alleles on chromosomes 4B and 7D in bread wheat

Dasturani, Esmaeil; Zaynali Nezhad, Khalil; Soltani Najafabadi, Masood; Pahlevani, Mohammadhadi; Soltanlo, Hassan; Bagherikia, Saeed

doi:10.52547/pgr.8.1.9

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Volume 8, Issue 1 (2021)

pgr 2021, 8(1): 133-150

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Determination of haplotype variation and relationship between desirable agronomic traits and microsatellite alleles on chromosomes 4B and 7D in bread wheat

Esmaeil Dasturani

, Khalil Zaynali Nezhad ^*

, Masood Soltani Najafabadi

, Mohammadhadi Pahlevani

, Hassan Soltanlo

, Saeed Bagherikia

Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran , zaynalinezhad@gau.ac.ir

Abstract: (10001 Views)

The aim of this study was to determine the haplotype groups and identify the specific alleles associated with desirable agronomic characteristics in bread wheat. For this purpose, 42 local bread wheat genotypes belong to Iran region and nine commercial cultivars along with Chinese Spring variety (reference genotype) were cultivated in the format of augmented design and evaluated based on their 13 phenotypic traits. The results of descriptive statistics showed that awn length and day to flowering had the highest and lowest phenotypic coefficient of variation, respectively. Eight microsatellite markers were used to investigate the haplotype variation of QTLs associated with phenotypic traits located on wheat chromosomes 4B and 7D. The result showed that the genotypes were classified into 13 and 6 haplotype groups according to the allelic comparison with the reference genotype on chromosome 4B and 7D, respectively. In order to investigate the relationship between traits and markers, analysis of variance was performed based on completely randomized design with unequal numbers of replications for each marker. In general, of the 13 traits studied, there was a statistically significant linkage for eight traits and for the three traits, an allele-specific was introduced simultaneously. If the breeders are interested in genotype selection that simultaneously have three desirable characteristics such as early anthesis, semi-dwarfing and a greater number of grains per spike, they can use an allele-specific (153 bp) of Xgwm149-4B marker.

Keywords: Phenotypic traits, Marker-assisted selection, Landrace wheat, Allele-specific marker

Full-Text [PDF 721 kb] (1393 Downloads)

Type of Study: Research | Subject: Molecular genetics

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‎ 10.52547/pgr.8.1.9

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Dasturani E, Zaynali Nezhad K, Soltani Najafabadi M, Pahlevani M, Soltanlo H, Bagherikia S. Determination of haplotype variation and relationship between desirable agronomic traits and microsatellite alleles on chromosomes 4B and 7D in bread wheat. pgr 2021; 8 (1) :133-150
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