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:: دوره 7، شماره 1 - ( 1399 ) ::
جلد 7 شماره 1 صفحات 144-127 برگشت به فهرست نسخه ها
شناسایی، تعیین توالی و ارزیابی پایداری بیان هشت ژن مرجع گیاه زعفران (.Crocus sativus L)
سید سجاد سهرابی ، سید محسن سهرابی* ، سید کریم موسوی ، محسن محمدی
گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه لرستان، خرم‌آباد ، sohrabi.sa@fa.lu.ac.ir
چکیده:   (8234 مشاهده)
زعفران (.LCrocus sativus ) ارزشمندترین و گران‌ترین ادویه در سطح جهان است. کلاله‌های این گیاه منبع آپوکاروتنوئیدهای با ارزشی مانند کروسین، پیکروکروسین و سافرانال هستند. مطالعات ترنسکریپتومی و بیانی ژن‌ها یکی از مهم‌ترین مراحل در بررسی تولید متابولیت‌های ثانویه‌ در گیاهان هستند. یکی از پیش‌نیازهای مهم در این گونه مطالعات، شناسایی ژن‌های مرجع قابل‌اعتماد و پایدار به‌منظور نرمال‌سازی بیان سایر ژن‌ها است. در پژوهش حاضر، با استفاده از ترنسکریپتوم گیاه زعفران هشت ژن مرجع شناسایی و تعیین توالی شد، سپس میزان پایداری بیان آن‌ها با استفاده از روش‌‌های غیرپارامتریک مورد بررسی قرار گرفت. نتایج تکثیر و توالی‌یابی، جداسازی صحیح هشت ژن مرجع Actin، EF1، GAPDH، H3، MDH، TBP، UBC و UBQ را از گیاه زعفران نشان داد. بررسی میزان پایداری بیان ژن‌های مرجع نشان داد که ژن‌های MDH و UBQ به‌ترتیب بیشترین میزان پایداری را بین بافت‌های مختلف گیاه زعفران داشته و کمترین میزان پایداری مربوط به ژن‌ TBP بود. در این پژوهش توالی هشت ژن مرجع در زعفران جداسازی و پایداری بیان آن‌ها برای اولین‌بار در بافت‌های مختلف این گیاه سنجیده شد. ژن‌های مرجع شناسایی شده در این پژوهش می‌توانند به‌عنوان ژن‌های پایدار برای نرمال‌سازی بیان ژن‌ها در مطالعات ترنسکریپتومی گیاه زعفران به‌کار برده شوند.
واژه‌های کلیدی: آماره‌های غیرپارامتریک، پایداری بیان ژن، ترنسکریپتوم، زعفران، ژن‌های مرجع
متن کامل [PDF 663 kb]   (1116 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: ژنتیک مولکولی
پذیرش: 1399/10/23
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Sohrabi S S, Sohrabi S M, Mousavi S K, Mohammadi M. Identification, Sequencing and Stability Evaluation of Eight Reference Genes in Saffron (Crocus sativus L.). pgr 2020; 7 (1) :127-144
URL: http://pgr.lu.ac.ir/article-1-177-fa.html

سهرابی سید سجاد، سهرابی سید محسن، موسوی سید کریم، محمدی محسن. شناسایی، تعیین توالی و ارزیابی پایداری بیان هشت ژن مرجع گیاه زعفران (.Crocus sativus L). پژوهش های ژنتیک گیاهی. 1399; 7 (1) :127-144

URL: http://pgr.lu.ac.ir/article-1-177-fa.html



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