[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 7, Issue 1 (2020) ::
pgr 2020, 7(1): 33-46 Back to browse issues page
Evaluation and Comparison of the Efficiency of Different Molecular Markers in Estimating Genetic Distance of Different Persian Oak (Quercus brantii Lindi.) Populations in Lorestan Province, Iran
Reza Mir Drikvand * , Kamran Samiei
Islamic Azad University, Khorramabad, Iran , mirderikvand@khoiau.ac.ir
Abstract:   (6272 Views)
Estimation of genetic diversity and evaluation of plant germplasm is the most important step in collection and management of plant genetic resources. Also, comparison of different DNA-based genetic markers in diversity evaluation and then advising the most efficient markers is very important. In order to investigate genetic variation among Persian oak (Quercus brantii Lindi.) populations of Lorestan province (Iran), 20 genotypes were collected from different geographical and climatic regions. After DNA extraction, polymerase chain reactions (PCR) were used for study of polymorphism using three markers including ISJ, ISSR and SCoT. Genotyping was performed using the polymorphic bands obtained from all three markers separately, and also by combining the data of three markers. PCR results of the primers showed 91 polymorphic bands with an average of 71% per locus. The ISSR marker with 44 bands had the most polymorphic bands. Genotypes were discriminated by ISJ, ISSR and SCoT markers in 5, 6 and 5 groups, respectively, and using the combined data of three markers, genotypes were classified in 5 groups (each group included more than one genotype) and 3 group (each group included one genotype).  The results showed that the obtained clustering by different markers were nearly consistent with clustering of genotypes based on the climatic origin of genotypes. The most similarity between the groupings was between ISJ and ISSR markers with 89%. Overall, the results indicated the usefulness of markers used to estimate genetic distances between different oak communities.
Keywords: Persian oak, Genetic diversity, Zagros, Molecular markers
Full-Text [PDF 749 kb]   (1152 Downloads)    
Type of Study: Research | Subject: Molecular genetics
Accepted: 2021/01/2
References
1. Alikhani, L., Shafie Rahmani, M. Shabanian, N., Badakhshan, H. and Khub, A.K. (2014). Genetic variability and structure of Quercus brantii assessed by iSSR, IRAP and SCoT marker. Gene, 552: 176-183.
2. Ardi, M., Rahmani, F. and Siami, A. (2012). Genetic variation among Iranian oaks (Quercus spp.) using random amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology, 11(45): 10291-10296.
3. Aziznia, B., Badakhshan, H., Javadi, T. and Zamani, S. (2020). Assessment of diversity in barley genotypes (Hordeum vulgare L) based on beta-glucan content and ISSR markers. Plant Genetic Researches, 6(2): 97-110 (In Persian).
4. Batos, B., Jovanovic, D.S. and Milkovic, D. (2014). Spatial and temporal variability of flowering in the Oak (Quercus robur L.). Izvorni Znanstveni Clinic, 7: 371-379.
5. Bruschi, P., Vendramini, G.G., Bussotti, F. and Grossoni, P. (2000). Morphological and molecular diferentiation between Quercus petraea (Matt.) Liebl.and Quercus pubescens willd. (Fagaceae) in northern and central Italy. Annals of Botany, 85: 325-333.
6. Carabeo, M., Simeone, M.C., Cherubini, M., Mattia, C., Chiocchini, F., Bertini, L., Caruso, C., Mantia, T.L., Villani, F. and Mattioni, C. (2017). Estimating the genetic diversity and structure of Quercus trojana Webb populations in Italy by SSRs: implications for management and conservation. Canadian Journal of Forest Researches, 47: 331-339.
7. Conte, L., Cott, C. and Cristofolini, G. (2007) Molecular evidence for hybrid origin of Quercus crenata Lam. (Fagaceae) from Q. cerris L. and Q. suber L. Plant Biosystems, 141: 181-193. [DOI:10.1080/11263500701401463]
8. Doyle, J.J. and Doyle J.L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.
9. Erfanifard, Y., Feghhi, J., Zobeiri, M. and Namiranian, M. (2009). Spatial pattern analysis in Persian oak (Quercus brantii var. persica) forests on B & W aerial photographs. Environmental Monitoring Assessment, 150: 251-259.
10. Geburek, T. and Konrad, H. (2008). Why the conservation of forest genetic resources has not worked? Conserved Biology, 22(2): 267-274.
11. Gheitarani, B., Erfani-Moghadam, J. and Fazeli, A. (2020). Evaluation of genetic diversity among some common Fig using RAPD and ISSR molecular markers. Plant Genetic Researches, 6(2): 43-54 (In Persian).
12. Grattapaglia, D., Plomion, C. Kirst, M. and Sederoff, R.R. (2009) Genomics of growth traits in forest trees. Current Opinion Plant Biology, 12:148-156. [DOI:10.1016/j.pbi.2008.12.008]
13. Jose-Maldia, L.S. Matsumoto, A., Ueno, S., Kanazashi, A., Kanno, M., Namikawa, K., Yoshimaru, H. and Tsumura, Y. (2017). Geographic patterns of genetic variation in nuclear and chloroplast genomes of two related oaks (Quercus aliena and Q. serrata) in Japan: implications for seed and seedling transfer. Tree Genetics and Genomes, 13:121.
14. Marakli, S. (2018). A brief review of molecular markers to analysis medicinally important plants. Journal of Life Sciences and Biotechnology, 1(1): 29-36.
15. Müller, M. and Gailing, O. (2018). Characterization of 20 new EST-SSR markers for northern red oak (Quercus rubra L.) and their transferability to Fagus sylvatica L. and six oak species of section Lobatae and Quercus. Annals of Forestry Research, 61(2): 211-222.
16. Neophytou, C., Aravanopoulos, F.A., Fink, S. and Dounavi, A. (2010). Detecting interspecific and geographic differentiation patterns in two inter fertile oak species (Quercus petraea (Matt.) Liebl. and Q. robur L.) using small sets of microsatellite markers. Forestry Ecology Management, 259: 20262035.
17. Olfat, A.M. and Pourtahmasi, K. (2010). Anatomical Characters in tree oak species (Q. libani, Q. brantii and Q. insectaria) from Iranian Zagros mountains. Australian Journal of Basic Applied Science, 4(8): 3230-3237.
18. Porth, I. and El-Kassaby, Y.A. (2014). Assessment of the genetic diversity in forest tree populations using molecular markers. Diversity, 6: 283-295.
19. Ranjan, R.G. and Aparajita, S. (2010). Phylogenic study of twelve species of Phyllanthus originated from India through molecular for conservation. American Journal of Plant Science, 1: 32-37.
20. Rohlf, F.J. (1998). NTSYSpc Version 2.02. Numerical Taxonomy and Multivariate Analysis System. Applied Biostatistics Inc., Exeter Software, Setauket, New York, USA.
21. Saenz-Romero, C. and Tapia-Olivares, B.L. (2003). Pinus oocarpa isoenzymatic variation along an altitudinal gradient in Michoacan, Mexico. Silvae Genet, 52: 237-240.
22. Saha, R. (2018). Genetic diversity in Quercus leucotrichophora populations through RAPD markers. Environment and Biotechnology, 11(1): 97-101.
23. Shabanian, N., Havasi, A. and Mehrabi, A.A. (2016). Genetic differentiation in Persian oak (Quercus brantii) populations using genomic inter-microsatellite markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(1): 66-78.
24. Shabanian, T., Alikhani, L. and Rahmani, M.S. (2015a). Genetic and phenotypic diversity of Iranian oak populations in declining forests of northern Zagros using biochemical characteristics and molecular marker SCoT. Journal of Genetic Research and Breeding of Range and Forest Plants, 23(1): 13-39 (In Persian).
25. Shabanian, T., Alikhani, L., Rahmani, M. and Badakhshan, S. (2015b). Evaluation of genetic and phenotypic diversity of Quercus libani populations in declining forests of northern Zagros using SCoT molecular marker and morphological and biochemical characteristics. Journal of Wood and Forest Science and Technology Research, 22(4): 55-77 (In Persian).
26. Shamari, A., Mehrabi, A.A., Maleki, A. and Rostami, A. (2008). Study of genetic diversity structure of Iranian oak (Quercus brantii Lindi.) Populations in the middle Zagros using genomic microsatellite markers. Iranian Journal of Forestry, 9(4): 511-525 (In Persian).
27. Shi, X., Wen, Q., Cao, M., Guo, X. and Xu, L. (2017). Genetic diversity and structure of natural Quercus variabilis population in China as revealed by microsatellites markers. Forests, 8: 495.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mir Drikvand R, Samiei K. Evaluation and Comparison of the Efficiency of Different Molecular Markers in Estimating Genetic Distance of Different Persian Oak (Quercus brantii Lindi.) Populations in Lorestan Province, Iran. pgr 2020; 7 (1) :33-46
URL: http://pgr.lu.ac.ir/article-1-167-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 7, Issue 1 (2020) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.07 seconds with 39 queries by YEKTAWEB 4642