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:: دوره 6، شماره 1 - ( 1398 ) ::
جلد 6 شماره 1 صفحات 30-1 برگشت به فهرست نسخه ها
کاربرد نشانگرهای مولکولی DNA در به‌نژادی گیاهان
سیدعلی‌محمد میرمحمدی میبدی* ، پوراندحت گلکار
گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان ، maibody@cc.iut.ac.ir
چکیده:   (18464 مشاهده)
به‌نژادی با هدف بهبود کمی و کیفی گیاهان، طیف گسترده‌ای از روش‌ها و فنون را به کار گرفته است. نشانگرهای ملکولی از ابزارهایی هستند که چشم‌انداز جدیدی را برای پیشرفت‌های به‌نژادی گیاهان فراهم کرده‌‌اند. این مقاله مزایا و کاربردهای متنوع نشانگرهای مولکولی و بهره‌گیری از توان بالای پلی‌مورفیسم طبیعی موجود در داخل جوامع در تلفیق با توانمندی‌های روش‌های به‌نژادی گیاهی مرسوم را مرور کرده است. نشانگرهای مولکولی از عوامل محیطی تأثیرپذیری ندارند و فراوانی بالای آنها از نظر تعداد و تنوع بالای ساختاری آنها به عنوان بخشی از مزایای نشانگرها در تشخیص هویت، تعیین تنوع ژنتیکی گونه‌ها و بررسی روابط خویشاوندی گیاهان به شمار می‌رود. نشانگرها در کشف اطلاعات بیشتر در مورد حفظ کلکسیون‌های ذخایر ژنتیکی گیاهی، تشخیص واریته‌ها، مکان‌یابی و تعیین تعداد ژن‌های کنترل‌کننده صفات کمک می‌کنند. توالی‌یابی ژنوم، تهیه نقشه‌های فیزیکی و ژنتیکی، انگشت‌نگاری‌های ژنومی و به‌نژادی گیاهان به عنوان برخی از دیگر کاربردهای این ابزار در به‌نژادی گیاهی می‌باشد. کارایی بالای انتخاب به کمک نشانگر در انتخاب ژنوتیپ‌ها به عنوان والد تلاقی و انتخاب به کمک نشانگر در برنامه‌های به‌نژادی و انتخاب ژنومی تأکید شده است. فناوری‌های جدید فرصت‌های خوبی را برای یافتن الگوهای تنوع ژنتیکی برای پیشبرد برنامه‌های به‌نژادی ایجاد کرده‌اند. امروزه با توسعه سریع فناوری توالی‌یابی‌های نسل جدید، توالی‌یابی ژنومی و روش‌های با کارایی بالا برای نشانگرها، ایجاد نشانگرهای جدید مانند EST-SSR از Simple Sequence Repeats (SSR) و Single Nucleotide Polymorphisms (SNPs) را آسان کرده است. این نشانگرها می‌توانند با موفقیت در شتاب‌بخشی به تحقیقات و برنامه‌های به‌نژادی گیاهان زراعی به‌کار گرفته شوند.
واژه‌های کلیدی: انتخاب، به نژادی، تنوع ژنتیکی، نشانگر مولکولی
متن کامل [PDF 1372 kb]   (7479 دریافت)    
نوع مطالعه: پژوهشي |
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MirMohammadi Maibody S A M, Golkar P. Application of DNA Molecular Markers in Plant Breeding (Review article). pgr 2019; 6 (1) :1-30
URL: http://pgr.lu.ac.ir/article-1-141-fa.html

میرمحمدی میبدی سیدعلی‌محمد، گلکار پوراندحت. کاربرد نشانگرهای مولکولی DNA در به‌نژادی گیاهان. پژوهش های ژنتیک گیاهی. 1398; 6 (1) :1-30

URL: http://pgr.lu.ac.ir/article-1-141-fa.html



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