[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 5, Issue 2 (2019) ::
pgr 2019, 5(2): 17-28 Back to browse issues page
Study on Phylogenetic Relationship among some of Iranian Wild Almond Species using Sequences of ITS1-5.8S rDNA-ITS2 Region and Chloroplastic trnL
Seyedeh Zahra Hosseini , Behroz Shiran , Mohammad Ali Ebrahimi *
Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran , ma_ebrahimi@pnu.ac.ir
Abstract:   (13868 Views)
Phylogenetic relations among 12 wild species of almonds, one cultivated almond and one species of peach were investigated by using of ITS1-5.8S rDNA-ITS2 sequences and trnL region of chloroplast DNA. To do this, maximum-parsimony and neighbor joining analysis adopted. Results of ITS data showed that studied species of Prunus only divided in two groups but incapable to separate different sections. P. tenella showed more diverse genetic distance in compare to other studied species and it seems that this species does not belong to Amygdalus. Also, by using the ITS data it can be reported that Prunus is monophyletic. In this research, the genetic distances for each pair of species were determined and the average genetic distance between species shows only the lowest genetic distance within the genus. Therefore, Prunus is a single genus. Regarding its high similarity of trnL region in wild almond species, it can be reported that maternal ancestor of Prunus is the same and trnL is not optimum marker to separate species of almond.
Keywords: Phylogeny, ITS1-5.8S rDNA-ITS2, Prunus species, trnL chloroplast
Full-Text [PDF 865 kb]   (1793 Downloads)    
Type of Study: Research | Subject: Plant genetics
References
1. Baldwin, B.G. (1993). Molecular phylogenetic of Calycadenia (Compositae) based on ITS sequences of nuclear ribosomal DNA: chromosomal and morphological evolution reexamined. American Journal of Botany, 80: 222-238. [DOI:10.1002/j.1537-2197.1993.tb13792.x]
2. Baldwin, B.G., Sanderson, G.M.J., Porter, J.M., Wojciehowski, M.E., Campbell, C.S. and Donoghue, M.J. (1995). The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Annals of the Missouri Botanical Garden, 82: 247-277. [DOI:10.2307/2399880]
3. Bortiri, E., Oh, S.H., Jiang, J., Baggett, S., Granger, A., Weeks, C., Buckingham, M., Potter, D. and Parfitt, D.E. (2001). Phylogeny and systematics of Prunus (Rosaceae) as determined by sequence analysis of ITS and the chloroplast trnL-trnF spacer DNA. Systematic Botany, 26: 797-807.
4. Bortiri, E., Vanden, H. and Potter, D. (2006). Phylogeny and systematics of Prunus (Rosaceae) as determined by sequence analysis of ITS and the chloroplast trnL-trnF spacer DNA. Systematic Botany, 26: 797-807.
5. Bouhadida, M., Martin, J.P., Eremin, G., Pinochet, J., Moreno, M.A. and Gogorcena, Y. (2007). Chloroplast DNA diversity in Prunus and its implication on genetic relationships. Journal of the American Society for Horticultural Science, 132(5): 670-679. [DOI:10.21273/JASHS.132.5.670]
6. Browicz, K. (1969). Amygdalus. In: Rechinger, K.H. (ed.), Flora Iranica: Rosaceae, Akademische Druck und Verlagsanstalt, Graz. 166-187.
7. Gilani, S.A., Qureshi, R.A., Khan, A.M. and Potter, D. (2010). A molecular phylogeny of selected species of genus Prunus L. (Rosaceae) from Pakistan using the internal transcribed spacer (ITS) spacer DNA. African Journal of Biotechnology, 9(31): 4867-4872.
8. Kester, D.E. and Assay, R. (1975). Almond", In: Advance in fruit breeding (ed. Janick J. and J. N. moore), Purdu Univ. Press West Lafayette, Indiana, 387-419.
9. Kester, D.E., Gradziel, T.M. and Grassely, C. (1991). Almonds (Prunus), In: M. Moore and J.R. Ballington. Jr. (eds). Genetic resources of temperate fruit and nut crops. International Journal of Horticultural Science and Technology, 701-758. [DOI:10.17660/ActaHortic.1991.290.16]
10. Khanuja, S.P.S., Shasany, A.K., Darokar, M.P. and Kumar, S. (1999). Rapid Isolation of DNA from Dry and Fresh Samples of Plant Producing Large Amounts of Secondary Metabolites and Essential oils. Kluwer Academic Publishers, The Netherlands.
11. Khatamsaz, M. (1992). Flora of Iran. No. 6: Rosaceae, Research Institute of Forest and rangelands.
12. Lee, S. and Wen, J. (2001). A phylogenetic analysis of Prunus and the Amygdaloideae (Rosaceae) using ITS sequences of nuclear ribosomal DNA. American Journal of Botany, 88: 150-160. [DOI:10.2307/2657135]
13. Martınez-Gomez, P., Arulsekar S., Potter D. and Gradziel, T.M. (2003). An extended inter-specific gene pool available to peach and almond breeding :as char:acterized using simple sequence repeat (SSR) markers. Euphytica, 131: 313-322.
14. Potter, D., Gao, E., Oh, S.H. and Baggett, S. (1999). Molecular phylogenetic studies in Rosaceae. P. 39. in International Botanical Congress-Abstracts.
15. Potter, D., Lubyand, J.J. and Harrison, R.E. (2000). Phylogenetic relationships among species of ragaria (Rosaceae) inferred from non-coding nuclear and chloroplast DNA sequences. Systematic Botany, 25: 337-348. [DOI:10.2307/2666646]
16. Rahemi, A., Fatahi, R., Ebadi, A., Taghavi, T., Hassani, D., Gradziel, T., Folta, K. and Chaparro, J. (2012). Genetic diversity of some wild almonds and related Prunus species revealed by SSR and EST-SSR molecular markers. Plant Systematic and Evolution, 298: 173-192. [DOI:10.1007/s00606-011-0536-x]
17. Socias i Company, R. (1998). La taxonomie de l'amandier. Cahiers Options Mediterr, 33: 91-93.
18. Sorkheh, K, Shiran, B., Gradziel, T.M., Epperson, B.K., Martinez-Gomez, P. and Asadi, E. (2007). Amplified fragment length polymorphism as a tool for molecular characterization of almond germplasm: genetic diversity among cultivated genotypes and related wild species of almond, and its relationships with agronomic traits. Euphytica, 156: 327-344. [DOI:10.1007/s10681-007-9382-x]
19. Taberlet, P., Gielly, L., Pautou, G. and Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17(5): 1105-1109. [DOI:10.1007/BF00037152]
20. Vadafar, M., Attar, F. and Maroofi, H. (2010). Trichome micromorphology in drupe of Amygdalus L. (Rosaceae) from Iran. Acta Botanica Croatica, 69(1): 93-105.
21. Vadafar, M., Kazempour, S.H. and Attar, F. (2014). Molecular phylogeny of the genus Amygdalus (Rosaceae) based on nrDNA ITS and cpDNA trnS-trnG sequences. Turkish Journal of Botany, 38: 439-452. [DOI:10.3906/bot-1303-46]
22. Wen, J., Berggren, S.T., Lee, C.H., Ickert-Bond, S., Yi, T.S., Yoo, K.O., Xie, J., Shaw, D. and Potter, D. (2008). Phylogenetic inferences in Prunus (Rosaceae) using chloroplast ndhF and nuclear ribosomal ITS sequences. Journal of Systematics and Evolution, 46(3): 322-332.
23. Yazbek, M. And Oh S.H. (2013). Peaches and almonds: phylogeny of Prunus subg. Amygdalus Rosaceae) based on DNA sequence and morphology. Plant Systematics and Evolution, 299: 1403-1418. [DOI:10.1007/s00606-013-0802-1]
24. Murray, M.G. and Thompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19): 4321-4325. [DOI:10.1093/nar/8.19.4321]
Send email to the article author



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseini S Z, Shiran B, Ebrahimi M A. Study on Phylogenetic Relationship among some of Iranian Wild Almond Species using Sequences of ITS1-5.8S rDNA-ITS2 Region and Chloroplastic trnL. pgr 2019; 5 (2) :17-28
URL: http://pgr.lu.ac.ir/article-1-127-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 5, Issue 2 (2019) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.07 seconds with 38 queries by YEKTAWEB 4657