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:: Volume 5, Issue 1 (2018) ::
pgr 2018, 5(1): 55-62 Back to browse issues page
Phylogenetic Analysis of some Iranian Chrysanthemum morifolium Cultivars, using Internal Transcribed Spacer (ITS) Molecular Markers
Mitra Shahbazi , Farhad Nazarian-Firouzabadi * , Omid Ali Akbarpour
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Irna , nazarian.f@lu.ac.ir
Abstract:   (11531 Views)
Chrysanthemum (Chrysanthemum morifolium) is one of the most important ornamental plants which plays a significant role in the development of gardening industry in the world. The knowledge of genetic diversity is one of the prerequisite criteria for Chrysanthemum breeding with important economic goals. Molecular markers have a significant share in elucidation of inter and intra species genetic diversity. To this end, genetic diversity of a number of Iranian cultivars was molecularly investigated by sequencing a part of rDNA, using ITS4 and ITS5 primers. Genetic distance between Chrysanthemum cultivars ranged from 0.05 to 10.15, demonstrating the power of ITS region in revealing the genetic diversity among cultivars of morifolium, suggesting Iranian cultivars have been genetically improved from morifolium species. Genetic diversity assessment of Iranian Chrysanthemum cultivars demonstrated that presumably inter, intra species or even inter population hybridization may have been involved in creating enormous genetic diversity among Chrysanthemum cultivars.
Keywords: Genetic diversity, Chrysanthemum morifolium, Molecular marker, ITS
Full-Text [PDF 569 kb]   (1791 Downloads)    
Type of Study: Research | Subject: Plant genetics
Accepted: 2019/03/31
References
1. Agarwal, M., Shrivastava, N. and Padh, H. (2008). Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Reports, 27: 617-631. [DOI:10.1007/s00299-008-0507-z]
2. Anderson, N.O. (2006). Flower Breeding and Genetics: Issues, Challenges and Opportunities for the 21st Century. Springer Science & Business Media, Dordrecht, NL.
3. Baliyan, D., Sirohi, A., Kumar, M., Kumar, V., Malik, S., Sharma, S. and Sharma, S. (2014). Comparative genetic diversity analysis in chrysanthemum: A pilot study based on morpho-agronomic traits and ISSR markers. Scientia Horticulturae, 167: 164-168. [DOI:10.1016/j.scienta.2013.12.029]
4. Da Silva, J.A.T. (2003). Chrysanthemum: advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotechnology Advances, 21: 715-766. [DOI:10.1016/S0734-9750(03)00117-4]
5. Da Silva, J.A.T. (2004). Ornamental chrysanthemums: improvement by biotechnology. Plant Cell, Tissue and Organ Culture, 79: 1-18. [DOI:10.1023/B:TICU.0000049444.67329.b9]
6. Huang, Y., An, Y.M., Meng, S.Y., Guo, Y.P. and Rao, G.Y. (2017). Taxonomic status and phylogenetic position of Phaeostigma in the subtribe Artemisiinae (Asteraceae). Journal of Systematics and Evolution, 55: 426-436. [DOI:10.1111/jse.12257]
7. Kia Mohammadi, F., Abdoosi, V., Moradi, P., Shafiei, M.R. and Arab, S. (2011). Phylogenetic analysis of some iranian inbreeding Chrysanthemum cultivars using morphological traits. Agronomy and plant breeding. Agronomy and Plant Breeding, 8: 43-45.
8. Liu, P.L., Wan, Q., Guo, Y.P., Yang, J. and Rao, G.Y. (2012). Phylogeny of the genus Chrysanthemum L.: evidence from single-copy nuclear gene and chloroplast DNA sequences. PloS One, 7: e48970. [DOI:10.1371/journal.pone.0048970]
9. Luo, C., Chen, D., Cheng, X., Zhao, H. and Huang, C. (2017). Genome size estimations in Chrysanthemum and correlations with molecular phylogenies. Genetic Resources and Crop Evolution, 64: 1451-1463. [DOI:10.1007/s10722-016-0448-2]
10. Ma, Y.P., Chen, M.-M., Wei, J.X., Zhao, L., Liu, P.L., Dai, S.L. and Wen, J. (2016). Origin of Chrysanthemum cultivars-Evidence from nuclear low-copy LFY gene sequences. Biochemical Systematics and Ecology, 65: 129-136. [DOI:10.1016/j.bse.2016.02.010]
11. Masuda, Y., Yukawa, T. and Kondo, K. (2009). Molecular phylogenetic analysis of members of Chrysanthemum and its related genera in the tribe Anthemideae, the Asteraceae in East Asia on the basis of the internal transcribed spacer (ITS) region and the external transcribed spacer (ETS) region of nrDNA. Chromosome Botany, 4: 25-36. [DOI:10.3199/iscb.4.25]
12. Nadeem, M.A., Nawaz, M.A., Shahid, M.Q., Doğan, Y., Comertpay, G., Yıldız, M., Hatipoğlu, R., Ahmad, F., Alsaleh, A. and Labhane, N. (2018). DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnology & Biotechnological Equipment, 32: 261-285. [DOI:10.1080/13102818.2017.1400401]
13. Nazarian-Firouzabadi, F. (2009). Principles of Plant Genetics and Breeding. Lorestan University Press, Khorramabad, IR.
14. Patwardhan, A., Ray, S. and Roy, A. (2014). Molecular markers in phylogenetic studies-a review. Journal of Phylogenetics and Evolutionary Biology, 2(2):1-9.
15. Roein, Z., Asil, M.H., Sabouri, A. and Dadras, A.R. (2014). Genetic structure of Chrysanthemum genotypes from Iran assessed by AFLP markers and phenotypic traits. Plant Systematics and Evolution, 300: 493-503. [DOI:10.1007/s00606-013-0898-3]
16. Salehan, S.M. and Nazarian Firouzabadi, F. (2017). Phylogenetic analysis of chrysanthemum morifolium cultivars by rpoC chloroplastic gene sequencing and morphological traits. Plant Genetic Researches, 4: 89-103. [DOI:10.29252/pgr.4.1.89]
17. Schilling, E.E. and Panero, J.L. (2011). A revised classification of subtribe Helianthinae (Asteraceae: Heliantheae) II. Derived lineages. Botanical Journal of the Linnean Society, 167: 311-331. [DOI:10.1111/j.1095-8339.2011.01172.x]
18. Sonboli, A., Stroka, K., Osaloo, S.K. and Oberprieler, C. (2012). Molecular phylogeny and taxonomy of Tanacetum L.(Compositae, Anthemideae) inferred from nrDNA ITS and cpDNA trnH-psbA sequence variation. Plant Systematics and Evolution, 298: 431-444. [DOI:10.1007/s00606-011-0556-6]
19. Won, S.Y., Jung, J.A. and Kim, J.S. (2018). The complete chloroplast genome of Chrysanthemum boreale (Asteraceae). Mitochondrial DNA Part B, 3: 549-550. [DOI:10.1080/23802359.2018.1468225]
20. Zhao, H.B., Chen, F.D., Chen, S.M., Wu, G.S. and Guo, W.M. (2010). Molecular phylogeny of Chrysanthemum, Ajania and its allies (Anthemideae, Asteraceae) as inferred from nuclear ribosomal ITS and chloroplast trnL-F IGS sequences. Plant Systematics and Evolution, 284: 153-169. [DOI:10.1007/s00606-009-0242-0]
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Shahbazi M, Nazarian-Firouzabadi F, Akbarpour O A. Phylogenetic Analysis of some Iranian Chrysanthemum morifolium Cultivars, using Internal Transcribed Spacer (ITS) Molecular Markers. pgr 2018; 5 (1) :55-62
URL: http://pgr.lu.ac.ir/article-1-122-en.html


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Volume 5, Issue 1 (2018) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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