In this review, an updated checklist of spider diversity in Rajasthan, India is presented. A total of 173 spider species from 90 genera belonging to 25 families are listed with records/descriptions originating from only 20 out of 33 districts of Rajasthan. A total of 74 taxa recorded from various districts of Rajasthan were identified only up to generic level. The maximum number of spider species were recorded from Jodhpur district (72 species), followed by Ajmer (69 species), Bharatpur (66 species), Pali (63 species), Jaipur (58 species), Dholpur and Karauli (38 species each), Jhunjhunu and Sikar (31 species each), Sri Ganga Nagar (26 species), Hanumangarh (25 species), and Jaisalmer (26 species). A fewer number of species are known from other districts. Thus far, no faunal surveys of spiders have been conducted in 13 districts of Rajasthan. Most of the national parks and wildlife sanctuaries, forest areas, agricultural fields, human dwellings, etc. within the state still await intensive and extensive surveys to record the spider fauna.