Volume 5, Issue 2 (6-2023)
JAD 2023, 5(2): 72-84 |
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Jayarajashekara T S, Ganapathi P P, Channaveerappa H. Distribution of chitin and collagen in the structural organization of the freshwater jellyfish Limnocnida indica Annandale, 1912 (Hydrozoa: Limnomedusae: Olindiidae). JAD 2023; 5 (2) :72-84
URL: http://jad.lu.ac.ir/article-1-306-en.html
URL: http://jad.lu.ac.ir/article-1-306-en.html
1- Department of Molecular Biology, Yuvaraja’s College (Autonomous and a Constituent College of University of Mysore), University of Mysore, Mysuru-570 005, India
Abstract: (991 Views)
In this paper, a structural analysis of the rarely encountered hydrozoan medusa Limnocnida indica Annandale is discussed. This diploblastic, radially symmetrical organism has a body plan typical of hydrozoan medusae. In this study more emphasis was given to observation of the supporting components of its anatomical structures. Detailed observation on the distribution of supportive components including chitin, collagen, and noncellular fibers within the tissue organization has been carried out with the application of different types of staining methods. The neuromuscular arrangement at the rim of the umbrella and basal part of the gastrovascular cavity and the distribution of the neurons in the sub epithelial region of ectoderm have been analyzed both by staining and by scanning electron microscopy studies. Supporting blocks of chitin at the base of tentacles, along with statocysts, and ring-like deposition of chitin and collagen on the tentacles are documented. Distribution of collagen around radial canals in the mesoglea was found to be unique to this species. The mesoglea seems to be a storehouse for the macromolecules chitin and collagen.
Keywords: Body plan, chitin blocks, collagen fibers, diploblastic, neuromuscular, subepithelial nerve cells, unique collagen
Type of Study: Original Research Article |
Subject:
Species Diversity
Received: 2023/03/24 | Accepted: 2023/06/19 | Published: 2023/06/30
Received: 2023/03/24 | Accepted: 2023/06/19 | Published: 2023/06/30
References
1. Aannandale, N. (1911). The occurrence of freshwater (Limnocnida) in Indian streams. Nature, 87: 144. [DOI:10.1038/087144b0]
2. Aannandale, N. (1912). Preliminary description of a freshwater Medusa from the Bombay Presidency. Records of the Indian Museum, 7: 253-256. [DOI:10.5962/bhl.part.28235]
3. Agharkar, S. P. (1913). Further notes on the habits and distribution of Limnocnida indica. Records of Indian Museum, 9: 247-249. [DOI:10.26515/rzsi/v9/i4/1913/163660]
4. Birsal, N. R. (1994). Occurrence of Limnocnida indica (Annadale) in the Pandri River (Western Ghats, Karnataka, India), with a note on freshwater medusae of India. Journal of the Bombay Natural History Society, 91: 91-94.
5. Bo, M., Bavestrello, G., Kurek, D., Paasch, S., Brunner, E., Born, R., Galli, R., Stelling, A. L., Sivkov, V. N., Petrova, O. V., Vyalikh, D., Kummer, K., Molodtsov, S. L., Nowak, D., Nowak, J. and Ehrlich, H. (2012). Isolation and identification of chitin in the black coral Parantipathes larix (Anthozoa: Cnidaria). International Journal of Biological Macromolecules, 51: 129-137. [DOI:10.1016/j.ijbiomac.2012.04.016] [PMID]
6. Ceccopieri Cassandra, Joanna Skonieczna, Jan P. Madej. (2021). Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue. Journal of Veterinary Research, 65: 125-130. [DOI:10.2478/jvetres-2021-0008] [PMID] []
7. Coers, C. (1952). The vital staining of muscle biopses with methylene blue. Journal of Neurology, Neurosurgery and Psychiatry, 15: 211-215. [DOI:10.1136/jnnp.15.3.211] [PMID] []
8. D'mello, A. X. P., Sylvester, T. V., Ramya, V., Britto, F. P., Shetty, P. K. and Jasphin, S. (2016). Metachromasia and metachromatic dyes: a review. International Journal of Advanced Health Sciences, 2 (10): 12-17
9. Darling, P. S. (1935). Occurrence of Limnocnida in the Periyar Lake, Travancore. Nature, 135: 151. [DOI:10.1038/135151a0]
10. Davis, L. E. and Haynes, J. F. (1968). An ultrastructural examination of the mesoglea of Hydra. Cell Tissue Research, 92: 149-158. [DOI:10.1007/BF00335643] [PMID]
11. Edston, E. and Gröntoft, L. (1997). Saffron - A connective tissue counter stain in routine pathology. Journal of Histotechnology, 20: 123-125. [DOI:10.1179/his.1997.20.2.123]
12. Fritzsch, B and. Zakon, H. H. (1988). A simple, reliable and inexpensive silver stain for nerve fibers in Bleached Skin. Zeitschrift für Naturforschung C, 43c: 606-608. [DOI:10.1515/znc-1988-7-820] [PMID]
13. Ghaskadbi, S. (2020). Hydra: a powerful biological model. Resonance, 25 (9): 1196-1213. [DOI:10.1007/s12045-020-1039-2] []
14. Gravely, F. A. and Agharkar, S. P. (1912). Notes on the habits and distribution of Limnocnida indica, Annandale. Recordings of Indian Museum, 7: 399-403. [DOI:10.26515/rzsi/v7/i4/1912/163117]
15. Hora, S. L. (1926). On some interesting features of the fauna of the western Ghats. Journal of the Bombay Natural History Society, 31: 447-449.
16. Hroudova, M., Vojta, P., Strnad, H., Krejcik, Z., Ridl, J., Paces, J., Vlcek, C. and Paces, V. (2012). Diversity, phylogeny and expression patterns of pou and six homeodomain transcription factors in hydrozoan jellyfish Craspedacusta sowerbyi. PLoSOne, 7 (4): e36420 [DOI:10.1371/journal.pone.0036420] [PMID] []
17. Iyengar, H. D. R. and Venkatesh, K. (1955). Occurrence of the freshwater medusa Limnocnida indica Annandale, in Thunga river near Shimoga town, Mysore State. Journal of the Bombay Natural History Society, 53: 151.
18. Jaigopal Sharma and Rina Chakrabarti (2000). Seasonal occurrence of freshwater medusa Limnocnida indica Annandale (Cnidaria: Limnomedusae) in a lake associated with the river Yamuna, India. Aquatic Ecology, 34 (2): 205-20. [DOI:10.1023/A:1009986516652]
19. Jankowski, T. (2001). The freshwater medusae of the world-a taxonomic and systematc literature study with some remarks on other inland water jellyfsh. Hydrobiologia, 462 (1-3): 91-113. [DOI:10.1023/A:1013126015171]
20. Jones, S. (1951). On the occurrence of the freshwater medusa, Limnocnida indica Annandale, in the western drainage of the Sahyadris. Journal of the Bombay Natural History Society, 49: 799-801.
21. Joshi, M. V. and Tonapi, S. T. (1965). A new record of freshwater medusa from India. Current Science, 34: 665-666.
22. Junqueira, L. C., Bignolas, G. and Brentani, R. R. (1979). Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Journal of Histochemistry and Cytochemistry, 11 (4): 447-455. [DOI:10.1007/BF01002772] [PMID]
23. Kavya, M. K., Raghunandan, K. S., Padmanabha, B. and Channaveerappa, H. (2018). Comparative scanning electron microscopic study of antennae and hearing organ of two Indian mantids Mantis religiosa and Gongylus gongylodes. International Journal of Zoological Investigations, 4 (1): 73-80.
24. Kaya, M., Lelešius, E., Nagrockaitė, R., Sargin, I., Arslan, G., Mol, A., Baran, T., Can, E. and Bitim, B. (2015). Differentiations of chitin content and surface morphologies of chitins extracted from male and female grasshopper species. PLoSOne, 10 (1): e0115531. [DOI:10.1371/journal.pone.0115531] [PMID] []
25. Köll, P., Borchers, G. and Metzger, J. O. (1991). Thermal degradation of chitin and cellulose. Journal of Analytical and Applied Pyrolysis, 19: 119-129. [DOI:10.1016/0165-2370(91)80038-A]
26. Krishnamurthy, D. R. (1951). On the occurrence of the freshwater medusa in the Krishnarajasagar on the Cauvery. Journal of the Bombay Natural History Society, 50: 955-56.
27. Kumar, M. N. V. R. (2000). A review of chitin and chitosan applications. Reactive and Functional Polymers, 46: 1-27. [DOI:10.1016/S1381-5148(00)00038-9]
28. Lattouf, R., Younes, R., Lutomski, D., Naaman, N., Godeau, G., Senni, K. and Changotade, S. (2014). Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues. Journal of Histochemistry and Cytochemistry, 62 (10): 751-758. [DOI:10.1369/0022155414545787] [PMID]
29. Manna, R. K., Das, A. K., Singh, D. N. and Khan, M. F. (2005). Occurrence of fresh water jellyfish, Limnocnida indica (Annadale) in Hemavathi Reservoir and its limnological perspective. Indian Journal of Fisheries, 52 (4): 483-487.
30. Ramakryshna, P. A., Bhimachar, B. S. and Subramaniam, M. K. (1950). Occurrence of the freshwater medusa (Linocnida india) in south west India. Journal of the Bombay Natural History Society, 49: 318-319.
31. Rao, H. (1931). The supposed resting stage of Limnocnida indica Annandale. Nature, 127: 971. [DOI:10.1038/127971a0]
32. Rao, H. 1933 (1932). Further observations on the freshwater medusa, Limnocnida indica Annandale, Journal of the Bombay Natural History Society, 36: 210-217.
33. Riyas, A. and Kumar, A. B. (2017). Record of freshwater jellyfsh blooms of invasive Craspedacusta sowerbii Lankester, 1880 (Hydrozoa, Limnomedusae) from Kerala, India. Journal of Aquatic Biology, Fisheries, 5: 154-159.
34. Smita, B., Manalee, V., Surve, Anjali, A., Kshama, K., Neha, S., Deepak, M. and Anirban, B. (2017). Histochemical staining of collagen and identification of its subtypes by Picrosirius red dye in mouse reproductive tissues. Bio-protocol, 7 (21): e2592. [DOI:10.21769/BioProtoc.2592] [PMID] []
35. Sreenivasan, N. and Barton, J. (2021). Freshwater medusae Limnocnia indica Anandale 1911 in the Cauvery Wildlife Sanctuary, Dubare Reserve Forest and Shivanasamudram in Karnataka, India, with a commentary note on the exotic Craspedacusta sowerbii Lankester, 1880. Journal of Threatened Taxa, 13 (3): 18035-18038. [DOI:10.11609/jott.6609.13.3.18035-18038]
36. Thomas, P. A., Thomas, K. M., Kirupashanker, P. and Senthil Maharajan, V. (1991). Use of lactophenol cotton blue mounts of corneal scrapings as an aid to the diagnosis of mycotic keratitis. Diagnostic Microbiology and Infectious Disease, 219:14: 219-224. [DOI:10.1016/0732-8893(91)90035-E] [PMID]
37. Vandepas, L. E., Tassia, M. G., Halanych, K. M. and Amemiya, C. T. (2023). Unexpected distribution of chitin and chitin synthase across soft-bodied cnidarians. Biomolecules, 13: 777. [DOI:10.3390/biom13050777] [PMID] []
38. Yan, D., Li, Y., Liu, Y., Li, N., Zhang, X. and Yan, C. (2021). Antimicrobial properties of chitosan and chitosan derivatives in the treatment of enteric infections Molecules, 25, 26 (23): 7136. [DOI:10.3390/molecules26237136] [PMID] []
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