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Showing 6 results for Hosseini
Seyedeh Zahra Hosseini, Behroz Shiran, Mohammad Ali Ebrahimi,
Volume 5, Issue 2 (3-2019)
Abstract
Phylogenetic relations among 12 wild species of almonds, one cultivated almond and one species of peach were investigated by using of ITS1-5.8S rDNA-ITS2 sequences and trnL region of chloroplast DNA. To do this, maximum-parsimony and neighbor joining analysis adopted. Results of ITS data showed that studied species of Prunus only divided in two groups but incapable to separate different sections. P. tenella showed more diverse genetic distance in compare to other studied species and it seems that this species does not belong to Amygdalus. Also, by using the ITS data it can be reported that Prunus is monophyletic. In this research, the genetic distances for each pair of species were determined and the average genetic distance between species shows only the lowest genetic distance within the genus. Therefore, Prunus is a single genus. Regarding its high similarity of trnL region in wild almond species, it can be reported that maternal ancestor of Prunus is the same and trnL is not optimum marker to separate species of almond.
Seyedeh Zahra Hosseini, Ahmad Ismaili, Seyed Sajad Sohrabi,
Volume 5, Issue 2 (3-2019)
Abstract
Drought is a threaded factor in the world production and application of breeding methods could improve the tolerant and adapted cultivators under drought stress condition. In order to evaluate and determine the stress tolerance indices and identifion of tolerant genotypes to drought stress, 15 safflower genotypes were evaluated in a randomized complete block design with three replications in two conditions (stress and non-stress). Analysis of variance showed significant differences among genotypes for all traits, stress tolerance indices and yield in both conditions. Significant positive correlation was found between grain yield in the stress condition with indicators stress tolerance index, harmonic mean and geometric mean productivity indicating that these indices are suitable criteria for screening drought tolerant genotypes. No significant correlation was observed between Ys with tolerance index and mean productivity, hence they can be discarded as the desirable markers for identifying drought tolerant genotypes. In conclusion, using a graphical approach of three dimensional scatter plots, Principal component analysis and biplot analysis, two tolerant genotype (Syrian and Kino-76) were selected for future programs in stress and non-stress condition.
Seyede Minoo Mirarab Razi, Reza Shirzadian-Khorramabad2, Hossein Sabouri, Babak Rabiei, Hossein Hosseini Moghadam5,
Volume 6, Issue 1 (9-2019)
Abstract
Salinity is an important limiting factor in the production of more plants, including rice. Due to the limited amount of cultivated area, identification of tolerant genotypes to environmental stresses and especially salinity is very important. The aim of this study was to investigate the genetic diversity among 114 recombinant lines derived from the intersection of local Tarom × Khazar cultivars under non stress conditions and salinity levels of 8 dS/m in reproductive stage in a completely randomized design. Combined analysis of variance showed that the differences between lines was significant for all traits. Genotypic variation coefficients also showed that the highest genetic variation among the evaluated recombinant lines was related to the number of panicles per plant. In contrast, days to 50% flowering showed the least genetic variation among these lines. In non stress and stress conditions, the highest genotypic and phenotypic correlation coefficient was observed between grain yield and number of fill grain in seedlings. Based on the cluster analysis of grain yield, the lines were classified into four groups under normal conditions and were classified into three groups under salinity conditions. The third-party lines in both cases had a higher average than the overall average. In general, the results of this study showed that there is a significant genetic variation between the studied lines in terms of salt tolerance and this variety can be used in subsequent corrective programs. Accordingly, lines 83, 81, 56, 39, 37 and 89 were the most sensitive lines and lines 107, 101, 16, 100, 84, 98, 47, 32, 14, 29, 95, 63, 5, 49, 92 and 10 were the most tolerant lines to salinity stresses of 8 dS/m and they also had higher yields and yield components. Strained lines are proposed directly for cultivating saline or for transferring salt tolerance to commercial cultivars through future breeding programs.
Azam Moayedinezhad, Behrooz Mohammadparast, Ghasem Hosseini Salekdeh, Ehsan Mohsenifard, Mohammad Ali Nejatian,
Volume 6, Issue 1 (9-2019)
Abstract
MicroRNAs (miRNAs), as a group of non-coding small RNAs, play key roles in regulating the growth, development and response of plants to various stresses. In this study, the expression patterns of three drought responsive miRNAs (miR159c, miR160a,b and miR169v) were compared in both drought tolerant (Yaghuti) and drought sensitive (Bidanesefid) grapevine cultivars using qRT-PCR under drought stress conditions. For identification the potential regulatory elements in the promoter regions of investigated miRNAs, the upstream sequences of their pre-miRNAs were analyzed using PlantCARE database. Drought related motifs such as ABREs and ABSs were identified in the regulatory regions of investigated miRNAs. Three transcription factors related to Auxin and ABA signaling were identified as the most important target genes for investigated miRNAs. The expression patterns of studied miRNAs were different affected from miRNA kind and grapevine cultivar. So, the expression of miR159 remained unchanged in Bidanesefid and increased in Yaghuti cultivar under drought stress condition, but the expression of miR160 and miR169 changed reversely in both cultivars. Cultivar dependent expression showed that miRNA responses to drought stress are also different among very relative genotypes with different drought susceptibility. Generally, considering the role of the potential target genes of investigated miRNAs, it seems that the change in the expression of evaluated miRNAs ultimately leads to the better tolerate of drought stress in Yaghuti cultivar.
Shahla Hosseini, Mohammad Reza Rahgozar, Hedieh Badakhshan,
Volume 8, Issue 2 (3-2022)
Abstract
Genus Allium L. contains very taxonomically complex sections, especially the subgenus Melanocrommyum. The systematic position of the species in each section has been revised many times over time. In the present study, the relationship between 32 ecotypes belonging to 10 different species of Allium was investigated using ISSR markers. The nine primers used produced 166 polymorphic bands (average 18 bands per primer). Among the primers used, ISSR873 primer with 27 bands made the most, and ISSR4 primer with two bands had the lowest polymorphic bands. The PIC of the markers ranged from 0.04 to 0.43. Cluster analysis by UPGMA method based on molecular markers divided the studied ecotypes into four groups. The clustering and principal coordinate analysis results showed that most morphologically similar species were grouped in closed clusters. According to Dice similarity coefficient, the highest percentage of similarity was shown between Allium stipitatum and Allium saralicum ecotypes (72 percent) from the Melanocrommyum subgenus, and the lowest similarity was obtained between Allium tripedale and Allium iranicum ecotypes (12 percent). The ecotypes with the lowest similarity percentage belong to the subgenus Allium and Nectaroscordum, which are placed in separate clusters. Based on the results, the ecotypes of Pseudoprason, Melanocrommyum, and Procerallium sections showed the highest affinity. In general, it can be concluded that ISSR markers are useful for classifying Allium species and have sufficient potential for phylogenetic studies of species. In addition, due to significant genetic diversity among the studied ecotypes of wild Allium species, this diversity can be used in future breeding programs of crop.
Forough Joudaki, Ahmad Ismaili, Seyed Sajad Sohrabi, Seyedeh Zahra Hosseini, Hadi Ahmadi,
Volume 10, Issue 2 (2-2024)
Abstract
Gall oak (Quercus infectoria) is one of the extraordinary tree species with functional medicinal properties within the oak family. Various studies have confirmed the presence of numerous secondary metabolites with therapeutic properties in this plant. Despite the significance of gall oak, its genetic structure remains elusive. Therefore, unraveling the genetic structure of gall ok may provide valuable insights into its potential applications across diverse industries. MicroRNAs emerge as pivotal genetic elements implicated in the biosynthesis of crucial metabolites across a wide range of different plant species. Despite the significant role of miRNAs in plants, as of yet, no miRNAs have been reported in Q. infectoria.. Therefore, in the present study, after assembling the transcriptome of Q. infectoria, the conserved microRNAs were identified. Leaf and root samples of Q. infectoria were collected from trees in the Shineh region, and 2-year-old seedlings were grown from mature oaks in Khorramabad (Lorestan Province, Iran). Total RNA was extracted from roots and leaves using the Djami-Tchatchou method. After sequencing by the Illumina HiSeq 2500 platform and checking the quality of all the generated reads, the adapter sequences were removed, and the high-quality reads were assembled using Trinity package. To identify miRNAs and their target genes, all plant miRNAs sequences were downloaded from the miRbase database. The BLASTn algorithm was employed to identify the highest similarity between unigenes and mature plant miRNAs. Furthermore, BLASTx was used to search against the non-redundant proteins (NR) database to remove protein-coding unigenes. The investigation of miRNA second-structure prediction involved assessing the similarity between potential unigenes and mature miRNA sequences using the mfold web tool. Identification of miRNA target genes and gene ontology (GO) was performed using the psRNAtarget web-tool and OmicsBox software, respectively. Following a range of strict filtering criteria, four miRNAs belonging to conserved miRNAs families were identified, including qin-miR156, qin-miR399, qin-miR160, and qin-miR172. KEGG pathway analysis showed the target genes were involved in the citrate cycle pathway. Examining miRNA target genes in Q. infectoria and analyzing their interaction network, finally led to the identification of three hub genes. Identified miRNA target genes were associated with the biosynthesis of various enzyme groups, suggesting that most of miRNAs regulating hydrolases, transferases, and oxidoreductases. Given the role of microRNAs in regulating transcription factors and their impact on genes involved in secondary metabolite biosynthesis, future breeding programs in Q. infectoria may benefit from the potential of such regulatory elements as a guide and key.
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