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:: Volume 2, Issue 1 (2015) ::
pgr 2015, 2(1): 23-34 Back to browse issues page
Isolation of Linalool Synthase and Pinene Synthase Genes from Yarrow (Achillea millefolium L.) Medicinal Plant
Maryam Javedan Asl , Hamid Rajabi Memari * , Daryoosh Nabati Ahmadi , Afrasiyab Rahnama Ghahfarokhi
Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran
Abstract:   (22271 Views)

Yarrow (Achillea millefolium) is an herbaceous and perennial plant species which belongs to the Asteraceae family. Yarrow's essential oil has different compounds of monoterpene and sesquiterpens, which its main constituents are pinene and linalool. These compunds have anti-microbial and anti-pest activities and also can be used in the food industry, perfumery and cosmetics.The aim of the present study was to use the degenerate primers approach in order to isolate Pinene synthase and linalool synthase genes from Yarrow plant. Up to date, there is no any report on the availability of these genes in the world gene bank. In this investigation the total RNA was extracted from Yarrow then pinene synthase and linalool synthase genes were isolated, using degenerate primers and Polymerase Chain Reaction (PCR). PCR amplified two bands of 250 bp and 720 bp. The sequence data were compared with NCBI gene bank data. The results of diversity study among varieties and families based on Pis and Lis genes showed most similarity between Achillea and Artemisia plants. Also this similarity was seen between Asteraceae and Lamiaceae families and these families grouped together same group. These results also showed a relatively high similarity of Pis and Lis with some other plants which confirmed sequencing data.

Keywords: Degenerate primer, Achillea millefolium, Pinene synthase, Terpenes, Linalool synthase
Full-Text [PDF 679 kb]   (2918 Downloads)    
Type of Study: Research | Subject: Plant improvement
Accepted: 2016/05/12
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Javedan Asl M, Rajabi Memari H, Nabati Ahmadi D, Rahnama Ghahfarokhi A. Isolation of Linalool Synthase and Pinene Synthase Genes from Yarrow (Achillea millefolium L.) Medicinal Plant. pgr 2015; 2 (1) :23-34
URL: http://pgr.lu.ac.ir/article-1-40-en.html


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Volume 2, Issue 1 (2015) Back to browse issues page
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